pMyNT-kan
(Plasmid #84693)

• Purpose: (Empty Backbone) Protein expression in mycobacteria
• Depositing Lab: Matthias Wilmanns
• Publication: Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.

Backbone

• Vector backbone: pMyNT
• Backbone manufacturer: Wilmanns lab
• Backbone size (bp): 6902
• Modifications to backbone: The hygromycin resistance cassette in pMyNT was replaced by a kanamycin resistance cassette using Gibson cloning. The pMyNT backbone was amplified using the primers : 5’-gtacgctagttaactacgtcg and 5’-cgccccctctagctgatcac and the kanamycin resistance cassette from pMV306 was amplified using the primers 5’-gtgaatagaggtccgctaacaaagcgacgttgtgtctc and 5’-cagctagagggggcgctgattagaaaaactcatcgagcatcaaatg.
• Vector type: Bacterial Expression
• Promoter: Acetamidase
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGAGTCCACCATGAAGCACC
• 3′ sequencing primer: TTGATGACGAGCGTAATGGC

Resource Information

• Supplemental Documents:
  • pMyNT-kan.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry