|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36855||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5694
- Total vector size (bp) 7167
Modifications to backbonea hygromysin resistance gene was inserted to facilitate selection for stably transfected cells.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namefluorescent protein voltage sensor
Alt namesuper ecliptic pHluorin
Insert Size (bp)1473
MutationCi-VSP contains R217Q mutation; super ecliptic pHluorin contains A227D mutation
GenBank IDAB183035 AY533296
- Promoter simian CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GACGTAAATGGGCGGTAGGCGTG
- 3′ sequencing primer TTAAAAAACCTCCCACACCTC (Common Sequencing Primers)
This construct is composed of the S1-4 domain of the CiVSP voltage sensor. Super ecliptic pHluorin is inserted at position S249 in the CiVSP sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ArcLight-S249 was a gift from Vincent Pieribone (Addgene plasmid # 36855 ; http://n2t.net/addgene:36855 ; RRID:Addgene_36855)
For your References section:Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe. Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Neuron. 2012 Sep 6;75(5):779-85. 10.1016/j.neuron.2012.06.040 PubMed 22958819