236,085 results

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pcDNA3-ALK5 wt

Plasmid

#80876

Purpose

mammalian expression of ALK5 WT

Depositor

Aristidis Moustakas

Publication

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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R777-E283 Hs.ROCK1

Plasmid

#70567

Purpose

Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ROCK1 (ROCK1 Human)

Use

Gateway Cloning

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E283 Hs.ROCK1

Plasmid

#70567

Purpose

Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ROCK1 (ROCK1 Human)

Use

Gateway Cloning

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Publication

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Promoter

mouse oxytocin

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Publication

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Promoter

mouse oxytocin

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDH-EF1-copGFP-T2A-Puro

Plasmid

#72263

Purpose

Express copGFP and puromycin resistance gene under EF1 promoter

Depositor

Kazuhiro Oka

Insert

copGFP-T2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

elongation factor-1

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

25 Articles

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pCDH-EF1-copGFP-T2A-Puro

Plasmid

#72263

Purpose

Express copGFP and puromycin resistance gene under EF1 promoter

Depositor

Kazuhiro Oka

Insert

copGFP-T2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

elongation factor-1

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

25 Articles

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MSP2135

Plasmid

#72248

Purpose

Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, and D1135E

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MSP2135

Plasmid

#72248

Purpose

Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, and D1135E

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST-FRT/T0-Flag-BRCA1

Plasmid

#71117

Purpose

mammalian expression of Flag-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV/TO

Available since

Nov. 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDEST-FRT/T0-Flag-BRCA1

Plasmid

#71117

Purpose

mammalian expression of Flag-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV/TO

Available since

Nov. 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Publication

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Publication

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMito-rxRFP1.1

Plasmid

#67841

Purpose

redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized

Depositor

Huiwang Ai

Publication

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rxRFP1.1 and mitochondrial localization sequence

Tags

His Tag and mitochondrial localization sequence

Expression

Mammalian

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMito-rxRFP1.1

Plasmid

#67841

Purpose

redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized

Depositor

Huiwang Ai

Publication

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rxRFP1.1 and mitochondrial localization sequence

Tags

His Tag and mitochondrial localization sequence

Expression

Mammalian

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHoss1

Plasmid

#63158

Purpose

Suicide plasmid for efficient gene mutation in Gram-positive bacteria

Depositor

Attila Karsi

Publication

Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

Insert

anti-secY-Pxyl/tetO-tetR

Use

Suicide plasmid for gram-positive bacteria

Available since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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