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Plasmid#113097PurposeGeneral donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-miRFP703
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-miniXon_Luciferase
Plasmid#174660PurposePlasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.DepositorInsertminiXon cassette
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#84446PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.DepositorHas ServiceAAV1, AAV5, and AAV8InsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry C1 Omp25
Plasmid#157758PurposeExpression of mcherry-labelled mitochondrial outer membrane protein 25 in cellsDepositorInsertSYNJ2bp (SYNJ2BP Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL3-deltaTM
Plasmid#109025Purposestable lentiviral expression of cDNADepositorInsertATL3-deltaTM (ATL3 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHW110033
Plasmid#222441PurposeLevel 0, CDS module (AATG-GCTT) containing Cre recombinase.DepositorInsertCre recombinase
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DNA Topoisomerase 1B Vaccinia Virus
Plasmid#165510PurposeUsed in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.DepositorInsertDNA Topoisomerase 1B Vaccinia Virus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Str-KDEL_SBP-EGFP-Ecadherin
Plasmid#65286Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationFragmentPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA1-CH-mneo
Plasmid#51288Purposecontains the fully codon optimized ORF1 and ORF2 of the human L1RPDepositorInsertL1 (L1RE1 Human)
UseTagsmneoI indicator cassette containing an inverted n…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDule- Mj Acd A9
Plasmid#197651PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3662 HPV-16 E2
Plasmid#22457DepositorInsertE2 WT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG
Plasmid#168998PurposeExpresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseUnspecifiedTagsEGFP and mRFP1ExpressionMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST39
Plasmid#64009Purposeoriginal polycistronic cloning vector for plasmid suite (generation I)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pSel
Plasmid#195344PurposeSelection plasmid expressing non-fluorescent EGFP A111VDepositorInsertEGFP
UseCRISPRTagsExpressionMutationA111VPromoterJ23119Available sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThpR
Plasmid#190613PurposeExpresses GFP in response to tetrahydropapaverine in E. coliDepositorInsertTHP4.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN3-Sstr3
Plasmid#35623DepositorInsertSstr3 (Sstr3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only