167,564 results

1. pCYP64

   Plasmid

   #212897

   Purpose

   Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Mutation

   P450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…

   Promoter

   PcymRC

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ8079 - IL2RG GFP-P2A CLIP Donor

   Plasmid

   #202690

   Purpose

   CLIP donor template for integrating GFP into IL2RG locus.

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Insert

   CopGFP
   

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-ALK5 K232R

   Plasmid

   #80878

   Purpose

   mammalian expression of ALK5 K232R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K232R (kinase dead)

   Promoter

   CMV

   Available Since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2-sgCTL

   Plasmid

   #234771

   Purpose

   Non Targeting Control

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Non Targeting Control sgRNA
   

   Use

   Lentiviral

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84242

   Purpose

   Expresses GA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

   Plasmid

   #101282

   Purpose

   Lentiviral expression of shRNA and mouse E-cadherin -mCherry

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-cadherin
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Jan. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BI

   Plasmid

   #126475

   Purpose

   High-copy number vector for the co-expression of two genes in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRES-HMGA1

   Plasmid

   #13466

   Depositor

   Edward Whang

   Article

   Liau et al Cancer Res. 2006 Dec 15. 66(24):11613-22

   Insert

   HMGA1 (HMGA1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEFIRES-P-EGFP-ADRP

    Plasmid

    #87157

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    mCherry-R TTGGTCACCTTCAGCTTGG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rtn3S-GFP

    Plasmid

    #169035

    Purpose

    Express human Rtn3S in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3S (RTN3 Human)
    

    Tags

    acGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCMV-SS-antiFLAGscFv-TM

    Plasmid

    #198303

    Purpose

    Displays anti-FLAG scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiFLAGscFv-TM
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL5.0 E-cadherin shRNA/Ecad-tdTomato

    Plasmid

    #101278

    Purpose

    Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-cadherin
    

    Use

    Lentiviral

    Tags

    tdtomato

    Expression

    Mammalian

    Available Since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1ab-Prkd1

    Plasmid

    #139314

    Purpose

    Diacylglycerol biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
    

    Expression

    Mammalian

    Mutation

    T208A, N313D

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-VEGFR1-V5/HIS

    Plasmid

    #205616

    Purpose

    Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR1 receptor tyrosine kinase (FLT1 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. MG1655 ΔendA ΔrecA (DE3)

    Bacterial Strain

    #37854

    Depositor

    Kristala Prather

    Article

    Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

    Bacterial Resistance

    None

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only