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Addgene

pDONR-A-HYG
(Plasmid #29635)

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Item Catalog # Description Quantity Price (USD)
Plasmid 29635 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR201
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 4470
  • Vector type
    Gateway donor

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    DB3.1 (ccdB resistant)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    DelsGate
  • GenBank ID
    EU360963

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a DelsGate vector that contains a selectable marker for the transformation of Ascomycete fungi, a TrpC promoter-hygR cassette from pCT48 (kindly provided by L. Ciuffetti) was inserted in pDONR201.

This plasmid was created by linearizing pDONR201 with HpaI, dephosphorylating and ligating to the HpaI excised
pCT48 hygR cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR-A-HYG was a gift from David_L Andrews & Scott Gold (Addgene plasmid # 29635 ; http://n2t.net/addgene:29635 ; RRID:Addgene_29635)

  • For your References section:

    DelsGate, a robust and rapid gene deletion construction method. Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE. Fungal Genet Biol. 2008 Apr . 45(4):379-88. 10.1016/j.fgb.2007.11.001 PubMed 18248826
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