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Pmec-18 caspase-3 (p12)::nz [TU#813]
(Plasmid #16082)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16082 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95.75
  • Backbone manufacturer
    Andrew Fire Lab
  • Backbone size w/o insert (bp) 3880
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    caspase-3 (p12) :: nz
  • Alt name
    caspase-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    519
  • Entrez Gene
    CASP3 (a.k.a. CPP32, CPP32B, SCA-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    caspase-3 from MGC full length cDNA clone ID 4419175.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

caspase-3 (p12) fused to the antiparallel leucine-zipper domain NZ (ALKKELQANKKELAQLKWELQALKKELAQ). For listed sequence, 1-397bp = mec-18p; 410-718bp = caspase-3(p12); 746-787bp = intron; 824-916bp = NZ.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pmec-18 caspase-3 (p12)::nz [TU#813] was a gift from Martin Chalfie (Addgene plasmid # 16082 ; http://n2t.net/addgene:16082 ; RRID:Addgene_16082)
  • For your References section:

    Targeted cell killing by reconstituted caspases. Chelur DS, Chalfie M. Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8. 10.1073/pnas.0610877104 PubMed 17283333