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Pmec-18 cz::ced-3 (p17) [TU#807]
(Plasmid #16081)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16081 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Andrew Fire Lab
  • Backbone size w/o insert (bp) 3880
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    cz:: ced-3 (p17)
  • Alt name
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Entrez Gene
    ced-3 (a.k.a. CELE_C48D1.2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ced-3 (p17) from Ding Xue, University of Colorado, Boulder

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ced-3 (p17) fused to the antiparallel leucine-zipper domain CZ (AQLEKKLQALEKKLAQLEWKNQALEKKLAQ). For listed sequence, 1-397bp = Pmec-18; 417-506bp = CZ; 549-590bp = intron; 615-2042bp = ced-3(p17).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pmec-18 cz::ced-3 (p17) [TU#807] was a gift from Martin Chalfie (Addgene plasmid # 16081 ; ; RRID:Addgene_16081)
  • For your References section:

    Targeted cell killing by reconstituted caspases. Chelur DS, Chalfie M. Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8. 10.1073/pnas.0610877104 PubMed 17283333