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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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PIP4K2B gRNA (BRDN0001147019)

Plasmid

#77903

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP4K2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP4K2B (PIP4K2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRPPRC gRNA (BRDN0001147349)

Plasmid

#77774

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRPPRC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRPPRC (LRPPRC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001146932)

Plasmid

#77602

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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KIT gRNA (BRDN0001147662)

Plasmid

#77562

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIT

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

KIT (KIT Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAG2 gRNA (BRDN0001149343)

Plasmid

#77462

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAG2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAG2 (PRKAG2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LRRK2 gRNA (BRDN0001147881)

Plasmid

#77333

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NPR1 gRNA (BRDN0001148992)

Plasmid

#77303

Purpose

3rd generation lentiviral gRNA plasmid targeting human NPR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NPR1 (NPR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKD1 gRNA (BRDN0001144834)

Plasmid

#77230

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD1 (PRKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK2 gRNA (BRDN0001146369)

Plasmid

#76912

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK2 (RIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ITPKB gRNA (BRDN0001145261)

Plasmid

#76874

Purpose

3rd generation lentiviral gRNA plasmid targeting human ITPKB

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ITPKB (ITPKB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001147643)

Plasmid

#76761

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K12 gRNA (BRDN0001147398)

Plasmid

#76762

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K12

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K12 (MAP3K12 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK2A1 gRNA (BRDN0001146603)

Plasmid

#76672

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK2A1 (CSNK2A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP4K2A gRNA (BRDN0001148000)

Plasmid

#76576

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP4K2A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP4K2A (PIP4K2A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK19 gRNA (BRDN0001149215)

Plasmid

#76502

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK19

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK19 (CDK19 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK1 gRNA (BRDN0001145958)

Plasmid

#76464

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK1 (PAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K3 gRNA (BRDN0001145020)

Plasmid

#76405

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K3 (MAP4K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP4K3 gRNA (BRDN0001147653)

Plasmid

#76406

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP4K3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP4K3 (MAP4K3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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