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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pFBA

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pGPD

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pADH2

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-sTagRFP-TUBA1B

Plasmid

#207765

Purpose

Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMx/ATP8B1(E234Q)-HA

Plasmid

#209253

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Mutation

E234Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B1(E234Q)-HA

Plasmid

#209241

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Tags

HA

Expression

Mammalian

Mutation

E234Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Xpress-GBF1(E794K)

Plasmid

#206418

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Expression

Mammalian

Mutation

E794K

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGS

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA8

Plasmid

#196103

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196106, 196107, 196108

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFB2gRNA12

Plasmid

#196106

Purpose

Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196107, 196108

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB2 (Safb2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFB2gRNA13

Plasmid

#196107

Purpose

Contains guide RNA to 3' end of mouse SAFB2 gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196108

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB2 (Safb2 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2C

Plasmid

#202736

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (PIP4K2C, FKBP1A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2CD280K

Plasmid

#202737

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (D278K) (PIP4K2C, FKBP1A Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2BD278K

Plasmid

#202735

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (D280K) (PIP4K2B, FKBP1A Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2AD273K

Plasmid

#202733

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Expression

Yeast

Promoter

CUP1 promoter

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del698-725-dsRNAres_U

Plasmid

#147718

Purpose

Insect Expression of DmNot3-del698-725-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del698-725-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del698-725-F764E-dsRNAres_U

Plasmid

#147719

Purpose

Insect Expression of DmNot3-del698-725-F764E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del698-725-F764E-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-del723-745-dsRNAres_U

Plasmid

#147720

Purpose

Insect Expression of DmNot3-del723-745-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-del723-745-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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