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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,121 results for: gibson assembly

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Showing: 1101 - 1120 of 1121 results

  1. pGTPA-Gm

    Plasmid
    #171527
    Purpose
    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene
    Depositor
    Quanjiang Ji
    Publication
    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:
    Insert
    Cas12k gRNA, transposon donor cassette, gentamycin-resistant gene
    Use
    CRISPR
    Promoter
    Constitutive promoter J23119
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    171527-plasmid-map-sequence-id-340981

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  3. pmEmerald_I3

    Plasmid
    #231680
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.
    Depositor
    Torsten Wittmann
    Publication
    Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014
    Insert
    I3-01
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231680-plasmid-map-sequence-id-474350

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  12. pHRSV_CHARMS-V43D/E52K/R62E

    Plasmid
    #233940
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Publication
    Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.
    Insert
    CHARMS-V43D/E52K/R62E
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233940-plasmid-map-sequence-id-469200

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  14. pHRSV_CHARMS-1xA

    Plasmid
    #233924
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Publication
    Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.
    Insert
    CHARMS-1xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233924-plasmid-map-sequence-id-468361

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  16. pHRSV_CHARMS-3xA

    Plasmid
    #233926
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Publication
    Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.
    Insert
    CHARMS-3xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233926-plasmid-map-sequence-id-469198

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  18. pHRSV_CHARMS-5xA

    Plasmid
    #233928
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutation
    Depositor
    Marcus Taylor
    Publication
    Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.
    Insert
    CHARMS-5xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233928-plasmid-map-sequence-id-468363

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  19. pHRSV_CHARMS-S34Y

    Plasmid
    #233929
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Publication
    Lichtenstein et al Science. 2025 Apr 25;388(6745):415-422.
    Insert
    CHARMS-S34Y
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233929-plasmid-map-sequence-id-469194

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Showing: 1101 - 1120 of 1121 results