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Plasmid#219709PurposeLevel 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pSPCH:PhiC31:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I87-SPCHPromoter_GG0-1
Plasmid#219707PurposeLevel 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pSPCH-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyTagsExpressionMutationWT promoter with added golden gate linkersPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2
Plasmid#219691PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG1-Bxb1P-bbs1-PhiC31P-GG2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-sfGFP-DsRed
Plasmid#80811PurposeVector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertsfGFP-piggyBac-DsRed
UseUnspecifiedTagssfGFPExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-3xFLAG-DsRed
Plasmid#80820PurposeVector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xFLAG-piggyBac-DsRed
UseUnspecifiedTags3xFLAGExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-2xHA-DsRed
Plasmid#80822PurposeVector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert2xHA-piggyBac-DsRed
UseUnspecifiedTags2xHAExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK614
Plasmid#72387PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 4 silent mutations in codons 9-11 and 42…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK621
Plasmid#72388PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsHis6ExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK592
Plasmid#72386PurposeProduces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)DepositorInsertphosphoribosylamine-glycine ligase
UseTagsExpressionBacterialMutationcontains 3 silent mutations in codons 9-11 to fac…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLXIN2-EGFP
Plasmid#99204PurposeEGFP expressing bicistronic retroviral vectorDepositorInsertEGFP
UseRetroviral; Bicistronic retroviral expression vec…TagsExpressionMammalianMutationA Kozak sequence was inserted 5' to ATG to …PromoterAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJW1328
Plasmid#69488PurposesgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegansDepositorInsertsgRNA(F+E) targeting pha-1
UseTagsExpressionWormMutationPromoterR07E5.16 U6 promoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD4-mT2A-mutated_rpsL-BSD-F
Plasmid#182338PurposeTemplate plasmid for PCR amplification of initial recombineering cassetteDepositorInsertBSD
UseMouse Targeting; Flp / frtTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
Plasmid#44083DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)
Plasmid#44095DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC03 (pFA6a-TEV-6xGly-V5-His3MX)
Plasmid#44072DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)
Plasmid#44071DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)
Plasmid#44089DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF266 (pFA6a-PSc-6xGly-3xFlag-His3MX)
Plasmid#44065DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags3xFlag, 6xGly linker, ADH1 transcription terminat…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only