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CKMT1A gRNA (BRDN0001145999)

Plasmid

#75878

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT1A

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT1A,CKMT1B (CKMT1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGFR3 gRNA (BRDN0001146639)

Plasmid

#75729

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR3 (FGFR3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGFR3 gRNA (BRDN0001147930)

Plasmid

#75730

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGFR3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGFR3 (FGFR3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAP3K7 gRNA (BRDN0001148226)

Plasmid

#75694

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K7

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STRADB gRNA (BRDN0001146450)

Plasmid

#75494

Purpose

3rd generation lentiviral gRNA plasmid targeting human STRADB

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STRADB (STRADB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN2 gRNA (BRDN0001147750)

Plasmid

#77926

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN2 (PKN2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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TXK gRNA (BRDN0001145857)

Plasmid

#76358

Purpose

3rd generation lentiviral gRNA plasmid targeting human TXK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TXK (TXK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Publication

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP (F64L, S65T, V163A)-His3MX6

Plasmid

#53185

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Publication

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

HIs3MX6 (HIS3 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460 K193A

Plasmid

#33326

Depositor

Anthony Means

Publication

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, K193A (S3V, G96R, and P108L als…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460 D311A

Plasmid

#33325

Depositor

Anthony Means

Publication

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, D311A (S3V, G96R, and P108L als…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf117

Plasmid

#12805

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Publication

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VV.Orf117

Expression

Mammalian

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Publication

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA

Plasmid

#246864

Purpose

Express BFP-NLS-P2A-RSV P-1xALFATag@72AA (Pexo-ALFATag) for RSV imaging.

Depositor

Marvin Tanenbaum

Publication

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

BFP-NLS-P2A-RSV P-1xALFATag@72AA

Use

Lentiviral

Tags

BFP-NLS

Expression

Mammalian

Mutation

RSV phosphoprotein (P) contains a single ALFA Tag…

Promoter

SFFV

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-2N4Rtau C291A/C322A

Plasmid

#249556

Purpose

Recombinant protein synthesis of C291A/C322A mutant of human 2N4R Tau

Depositor

Jeff Kuret

Publication

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (2N4R) (MAPT Human)

Expression

Bacterial

Mutation

Cysteine 291 changed to Alanine, Cysteine 322 cha…

Available since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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huCof C139S-mRFP

Plasmid

#245969

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C139S

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C147A-mRFP

Plasmid

#245970

Purpose

Expression of cof with one of 4 cys mutated to ala .Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C147A

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C147S-mRFP

Plasmid

#245971

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C147S

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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