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MAP2K7 gRNA (BRDN0001149491)

Plasmid

#77822

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K7 (MAP2K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKM gRNA (BRDN0001145396)

Plasmid

#77633

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKM (PKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001148670)

Plasmid

#77564

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001146365)

Plasmid

#77565

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BMPR1B gRNA (BRDN0001145690)

Plasmid

#77488

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR1B (BMPR1B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1D gRNA (BRDN0001149348)

Plasmid

#76716

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1D

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1D (CSNK1D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BMPR2 gRNA (BRDN0001144883)

Plasmid

#76391

Purpose

3rd generation lentiviral gRNA plasmid targeting human BMPR2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BMPR2 (BMPR2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CG gRNA (BRDN0001149186)

Plasmid

#76334

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CG (PIK3CG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN1 gRNA (BRDN0001162227)

Plasmid

#76276

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN1 (PKN1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TYRO3 gRNA (BRDN0001146050)

Plasmid

#76228

Purpose

3rd generation lentiviral gRNA plasmid targeting human TYRO3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TYRO3 (TYRO3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001146152)

Plasmid

#76111

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP3K8 gRNA (BRDN0001146835)

Plasmid

#75923

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K8 (MAP3K8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HSPB8 gRNA (BRDN0001145453)

Plasmid

#75640

Purpose

3rd generation lentiviral gRNA plasmid targeting human HSPB8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HSPB8 (HSPB8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CCL2 gRNA (BRDN0001147233)

Plasmid

#77865

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCL2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CCL2 gRNA (CCL2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-C/pIRES puro Glue (H987-end)

Plasmid

#73458

Purpose

Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-Q986)

Plasmid

#73457

Purpose

Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only