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We narrowed to 12,321 results for: LEA

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Showing: 11481 - 11500 of 12321 results

  3. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222015-plasmid-map-sequence-id-457427

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  6. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Robert Gross
    Publication
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225487-plasmid-map-sequence-id-444518

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  7. pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

    Plasmid
    #221446
    Purpose
    Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus
    Depositor
    Daniel Durocher
    Publication
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Tags
    Nuclear Export Signal (NES) and eGFP
    Expression
    Mammalian
    Mutation
    NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…
    Promoter
    CMV
    Available since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221446-plasmid-map-sequence-id-437089

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  8. pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP

    Plasmid
    #221444
    Purpose
    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus
    Depositor
    Daniel Durocher
    Publication
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Tags
    3XFlag and Nuclear Export Signal (NES)
    Expression
    Mammalian
    Mutation
    NES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …
    Promoter
    CMV
    Available since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221444-plasmid-map-sequence-id-437502

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  10. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid
    #208971
    Purpose
    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208971-plasmid-map-sequence-id-415826

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  11. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  12. B-HeFSpCas9-A695Q

    Plasmid
    #207368
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A695Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    207368-plasmid-map-sequence-id-410902

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  13. B-HeFSpCas9-A926Q

    Plasmid
    #207370
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A926Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207370-plasmid-map-sequence-id-410164

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  14. B-HeFSpCas9-A1060R

    Plasmid
    #207372
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1060R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …
    Promoter
    CBh
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207372-plasmid-map-sequence-id-408921

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  15. B-HeFSpCas9-A1003K

    Plasmid
    #207371
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1003K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …
    Promoter
    CBh
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207371-plasmid-map-sequence-id-408916

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  16. B-HeFSpCas9-A848K

    Plasmid
    #207369
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A848K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207369-plasmid-map-sequence-id-408749

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  17. B-HeFSpCas9-A661R

    Plasmid
    #207367
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207367-plasmid-map-sequence-id-408748

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  18. B-HypaR-SpCas9

    Plasmid
    #126764
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A; N692A; M694A; Q695A; H698A; amino acids 10…
    Promoter
    CBh
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126764-plasmid-map-sequence-id-410886

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Showing: 11481 - 11500 of 12321 results