We narrowed to 15,981 results for: LEA

1. pRRL_U2AF1_S34F_mCherry

   Plasmid

   #84019

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34F

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP1174

   Plasmid

   #104162

   Purpose

   Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-BaLgp160

   Plasmid

   #100919

   Purpose

   Mammalian expression plasmid for Env from the BaL HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-NRF1-HA

   Plasmid

   #34708

   Depositor

   Raymond Deshaies

   Article

   Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

   Insert

   NRF1 (NFE2L1 Human)
   

   Use

   Retroviral

   Tags

   3XFlag and HA

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-NRF1

   Plasmid

   #34707

   Depositor

   Raymond Deshaies

   Article

   Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

   Insert

   NRF1 (NFE2L1 Human)
   

   Use

   Retroviral

   Tags

   3XFlag

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

   Plasmid

   #175524

   Purpose

   Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7 and Lyn11-SNAPf
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR-Ec611

   Plasmid

   #130872

   Purpose

   A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Expression

   Yeast

   Mutation

   I777K, L900S

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Orai1 CFP

   Plasmid

   #19757

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid

    #74315

    Purpose

    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish

    Depositor

    Stephen Smith, Martin Meyer

    Article

    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

    Inserts

    zebrafish PSD95
    DsRedExpress
    

    Tags

    EGFP

    Mutation

    Y599H and I793S (please see depositor comment bel…

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-Venus-CAAX

    Plasmid

    #87612

    Purpose

    Plasma membrane-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus-CAAX
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-DU422gp160

    Plasmid

    #100926

    Purpose

    Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422.1) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-CH111gp160

    Plasmid

    #123268

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-B41gp160

    Plasmid

    #123247

    Purpose

    Mammalian expression plasmid for Env from the B41 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (B41) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-eBFP2-NLS-pA

    Plasmid

    #183800

    Purpose

    AAV vector to express nuclear localized eBFP2 from CMV promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    eBFP2-NLS
    

    Use

    AAV

    Tags

    NLS

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PP1 7-300

    Plasmid

    #26566

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    PP1a (PPP1CA Human)
    

    Tags

    His6, TEV cleavage site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains only aa 7-330

    Available Since

    Dec. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. cr3Dd

    Plasmid

    #184862

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, bet, exo
    

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-AD8gp160

    Plasmid

    #123260

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-25711gp160

    Plasmid

    #111841

    Purpose

    Mammalian expression plasmid for Env from the 25711 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (25711.2) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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