We narrowed to 15,989 results for: LEA

1. pGBW-m4046959

   Plasmid

   #145722

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher002-oTri

   Plasmid

   #124662

   Purpose

   Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTri
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVB3011

   Plasmid

   #113389

   Purpose

   Galactose-inducible L-BC gag: cure killer

   Depositor

   Suzanne Sandmeyer

   Article

   Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

   Insert

   L-BC Gag
   

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Clomeleon

   Plasmid

   #203723

   Purpose

   A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

   Depositor

   George Augustine

   Article

   Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

   Insert

   cyan fluorescent protein linked yellow florescent protein
   

   Expression

   Bacterial and Mammalian

   Mutation

   Cyan Fluorescent Protein, Topaz Fluorescent Prote…

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Venus-StagRFP-AktAR

   Plasmid

   #173020

   Purpose

   Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Venus-StagRFP-AktAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM3

   Plasmid

   #154168

   Purpose

   Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5T7-LYCipi-ggpps

   Plasmid

   #122017

   Purpose

   Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Insert

   GGPP synthase
   

   Expression

   Bacterial

   Mutation

   codon optimized, truncated first 98 amino acids, …

   Promoter

   T7

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet_NADP-Snifit-nucl

   Plasmid

   #113918

   Purpose

   Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Expression

   Mammalian

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV U6+27

    Plasmid

    #25709

    Purpose

    for nuclear expression of small RNAs in mammalian cells

    Depositor

    David Engelke

    Article

    Good et al Gene Ther. 1997 Jan . 4(1):45-54.

    Insert

    U6 snRNP (LSM2 Human)
    

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. V2-MESA-35F-M-dCas9

    Plasmid

    #84506

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQL47 EGFP-VAMP-GST

    Plasmid

    #122971

    Purpose

    Reporter of botulinum toxin B cleavage

    Depositor

    Chandra Tucker

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    EGFP-VAMP3-GST
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pElectra2-LaminA

    Plasmid

    #179483

    Purpose

    Nuclear envelope labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-LaminA
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMM289

    Plasmid

    #46037

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A/K577M (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A/K577M exonuclease and helicase-dead mutant; …

    Promoter

    CMV2

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pBEL1284

    Plasmid

    #175960

    Purpose

    For expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Tags

    6xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM288

    Plasmid

    #46036

    Depositor

    Raymond Monnat

    Article

    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8

    Insert

    WRN E84A (WRN Human)
    

    Use

    Tre, bidirectional promoter

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    E84A exonuclease-dead mutant; S2G introduced duri…

    Promoter

    CMV2

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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