Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 15,824 results for: LEA

Showing: 11541 - 11560 of 15824 results

  1. pOCC114

    Plasmid
    #118904
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with TEV protease, two glycines
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118904-plasmid-map-sequence-id-232331

    Enlarge

  3. pGBW-m4046879

    Plasmid
    #149238
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149238-plasmid-map-sequence-id-357573

    Enlarge

  4. pET21a - APX

    Plasmid
    #89618
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Ascorbate dependent Tryparedoxin peroxidase
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89618-plasmid-map-sequence-id-176685

    Enlarge

  5. pGBW-m4046942

    Plasmid
    #145757
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145757-plasmid-map-sequence-id-292503

    Enlarge

  8. pcDNA3-Lyn-AktAR

    Plasmid
    #123356
    Purpose
    FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.
    Depositor
    Jin Zhang
    Article
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Insert
    Lyn-AktAR
    Use
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    123356-plasmid-map-sequence-id-307195

    Enlarge

  9. pGBW-m4046763

    Plasmid
    #145606
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145606-plasmid-map-sequence-id-298330

    Enlarge

  10. pGBW-m4046789

    Plasmid
    #145660
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145660-plasmid-map-sequence-id-298333

    Enlarge

  11. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

    Enlarge

  14. pGBW-m4046933

    Plasmid
    #145592
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145592-plasmid-map-sequence-id-292573

    Enlarge

  18. pGBW-m4136463

    Plasmid
    #149527
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149527-plasmid-map-sequence-id-300219

    Enlarge

  19. pGBW-m4046901

    Plasmid
    #145725
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145725-plasmid-map-sequence-id-297834

    Enlarge

  20. CXXC1

    Plasmid
    #32045
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC1 (CXXC1 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    CXXC domain (amino acids residues 161-222)
    Promoter
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 11541 - 11560 of 15824 results