We narrowed to 5,835 results for: pCas

1. pCAG/ATP10D(E215Q)-HA

   Plasmid

   #209248

   Purpose

   Mammalian expression of ATP10D mutant E215Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10D (ATP10D Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E215Q

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-CoChR-mRuby2-ST

   Plasmid

   #109131

   Purpose

   Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   CoChR-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Promoter

   osk

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-memRFP-3xPax7gRNA

   Plasmid

   #224569

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/ATP9A(E195Q)-HA

   Plasmid

   #209244

   Purpose

   Mammalian expression of ATP9A mutant E195Q

   Depositor

   Hye-Won Shin

   Article

   Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

   Insert

   ATP9A (ATP9A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E195Q

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-OSF-VPS15

    Plasmid

    #99326

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS15 (PIK3R4 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

11. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-mCherry2-mito

    Plasmid

    #164164

    Purpose

    Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2-mtActA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER50-SpCas9-ER50

    Plasmid

    #85448

    Purpose

    Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    ER50-SpCas9-ER50
    

    Use

    AAV

    Tags

    destabilized domain of estrogen receptor ligand b…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-CBE6a-IVT

    Plasmid

    #215833

    Purpose

    Template for in vitro transcription of CBE6a (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-OSF-BECN1

    Plasmid

    #99328

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    Beclin 1 (BECN1 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pCALNL-Rheb(S16H)

    Plasmid

    #194204

    Purpose

    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

    Depositor

    Angelique Bordey

    Article

    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    S16H

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-HA-Nrxn1α-LNS3

    Plasmid

    #128107

    Purpose

    Express HA tagged Nrxn1α-LNS3

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS3 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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