We narrowed to 15,824 results for: LEA

1. pcDNA-NLS-ZapCmR1.1

   Plasmid

   #59011

   Purpose

   Nuclear zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4046841

   Plasmid

   #145578

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHR-MCS-YFP-NLS

   Plasmid

   #145274

   Purpose

   Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MCS and YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Split-RESCUE-C

   Plasmid

   #170154

   Purpose

   AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   RESCUE-DDC-λN-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4136460

   Plasmid

   #149532

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ARB342

   Plasmid

   #124025

   Purpose

   Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a - SodB

   Plasmid

   #89613

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Superoxide dismutase B
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-SGTAP no-pA

   Plasmid

   #82599

   Purpose

   3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   SBP-TEV-2x protein G
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Glt1b

   Plasmid

   #98706

   Purpose

   mammalian expression of Glt1b

   Depositor

   Paul Rosenberg

   Article

   Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

   Insert

   Glt1b (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I348V and N365Y (please see depositor comments se…

   Promoter

   CMV

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA-FRT-FAK-kinase0

    Plasmid

    #59122

    Purpose

    FAK fret control sensor without FERM domain with no linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGK415-­phlTA2-­1E

    Plasmid

    #165970

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. V32M,L84M CoV-2 ORF8

    Plasmid

    #159934

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Use

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    V32M, L84M

    Promoter

    T7

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ptCMV-153/47-VR-NG

    Plasmid

    #50704

    Purpose

    Final destination vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    TALEN

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PHF13 (3O70)

    Plasmid

    #28154

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PHD finger protein 13 (PHF13 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    PHD-type zinc finger domain (residues 232-281).

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pGBW-m4136462

    Plasmid

    #149533

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046932

    Plasmid

    #145750

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4095421

    Plasmid

    #149524

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-745 d1063-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046728

    Plasmid

    #145739

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge