We narrowed to 15,989 results for: LEA

1. BPTI-[5,55]Gly14Ile38

   Plasmid

   #195059

   Purpose

   Thermal stability of BPTI

   Depositor

   Yutaka Kuroda

   Article

   Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

   Insert

   bovine pancreatic trypsin inhibitor
   

   Tags

   His tag and trpΔLE leader sequences

   Expression

   Bacterial

   Mutation

   Changed Alanine residues at position of 14 and 38…

   Promoter

   T7 promotor

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-nDsRedFL-intron

   Plasmid

   #177804

   Purpose

   An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   intron
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 Flag-NLS-Smad2

   Plasmid

   #14931

   Depositor

   Joan Massague

   Article

   Lo et al Nat Cell Biol. 1999 Dec . 1(8):472-8.

   Insert

   NLS-Smad2 (SMAD2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   full-length Smad2 is fused to a nuclear-localizat…

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. cr3Ec

   Plasmid

   #184860

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nuc-ABKAR

   Plasmid

   #61511

   Purpose

   Expression of nuclear AMPK biosensor

   Depositor

   Takanari Inoue, Jin Zhang

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Tags

   NLS (see comments)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

   Plasmid

   #216377

   Purpose

   Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

   Depositor

   William Weis

   Insert

   LDL receptor related protein 6 (LRP6 Human)
   

   Use

   Baculovirus

   Tags

   9xHis and HA signal sequence-FLAG-3C cleavage sit…

   Expression

   Insect

   Promoter

   Polyhedrin (LRP6)/p10 (MESD)

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046760

   Plasmid

   #145600

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. PXWABS62(E11-TEV)

   Plasmid

   #123157

   Purpose

   For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   ParsR-ABS62, Pecf11

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. V2-MESA-35F-M-tTA

    Plasmid

    #84502

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-tTA
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4046955

    Plasmid

    #145616

    Purpose

    Bacterial Expression plasmid for RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. SeeSaw Reporter (SSR1.0)

    Plasmid

    #50392

    Purpose

    SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

    Depositor

    Pablo Huertas Sánchez

    Article

    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

    Insert

    SSR1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dendra2-LaminB1-10

    Plasmid

    #57728

    Purpose

    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211841

    Purpose

    Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA and mCherry

    Expression

    Plant

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEP110E

    Plasmid

    #97396

    Purpose

    binary construct of nuclear mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Tags

    Nucleus target

    Expression

    Plant

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046814

    Plasmid

    #145601

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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