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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCfuNca-Mito

Plasmid

#190819

Purpose

Mitochondria targeted GFP for diatoms

Depositor

Peter Kroth

Article

Sprecher et al Algal Research. 2023 May; Vol 72: 103136

Inserts

Diatom mitochondrial targeted GFP
Bleomycin resistance gene

Expression

Bacterial

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus-apCC-Di

Plasmid

#190711

Purpose

Encodes C-Venus followed by the antiparallel coiled-coil apCC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus-apCC-Di

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus

Plasmid

#190713

Purpose

Control plasmid encoding C-Venus, but without a coiled-coil to drive reconstitution.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-apCC-Di-C-Venus

Plasmid

#190709

Purpose

Encodes the antiparallel coiled-coil apCC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

apCC-Di-C-Venus

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT 3MYC SMASh

Plasmid

#182524

Purpose

C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Tags

3MYC

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT 3MYC SMASh

Plasmid

#182525

Purpose

C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSQHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Tags

3MYC

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al bioRxiv 2024.02.22.581357

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397C_ABE

Plasmid

#189648

Purpose

psa A Right _G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 C-ABE

Plasmid

#189650

Purpose

rbc L 294 Left_G1397 C-ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-5.1

Plasmid

#190589

Purpose

Lentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells

Depositor

Jakob Reiser

Article

Bialkowska et al BMC Genomics. 2005 Sep 4;6:113. doi: 10.

Inserts

NeoR transgene
artificial exon encoding BSD and EGFP

Use

Lentiviral

Promoter

Cellular and SV40

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2414

Plasmid

#175966

Purpose

Genetic complementation plasmid of LetAB with LetB(P701N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

P701N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2415

Plasmid

#175967

Purpose

Genetic complementation plasmid of LetAB with LetB(I703N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

I703N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2416

Plasmid

#175968

Purpose

Genetic complementation plasmid of LetAB with LetB(A705N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

A705N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2417

Plasmid

#175969

Purpose

Genetic complementation plasmid of LetAB with LetB(A706N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

A706N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2418

Plasmid

#175970

Purpose

Genetic complementation plasmid of LetAB with LetB(G707N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

G707N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2419

Plasmid

#175971

Purpose

Genetic complementation plasmid of LetAB with LetB(V708N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

V708N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2420

Plasmid

#175972

Purpose

Genetic complementation plasmid of LetAB with LetB(P717N)

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Expression

Bacterial

Mutation

P717N

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAB166

Plasmid

#101668

Purpose

Opto-T7RNAP*(563-F2), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB171

Plasmid

#101670

Purpose

Opto-T7RNAP*(69), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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