Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,379 results for: ABE8e

Showing: 1161 - 1180 of 1379 results

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YEE-NG-CBE

Plasmid

#227418

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Publication

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(5p)-WT

Plasmid

#215875

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-WT

Plasmid

#215876

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-WT

Plasmid

#215877

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-WT (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(3p)-WT

Plasmid

#215878

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a2(3p)-WT

Plasmid

#215879

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-99a-WT

Plasmid

#215880

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(5p)-I-type

Plasmid

#215882

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a1(3p)-I-type

Plasmid

#215883

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-589(5p)-I-type

Plasmid

#215884

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-589(3p)-I-type

Plasmid

#215885

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-22(3p)-I-type

Plasmid

#215886

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-376a2(3p)-I-type

Plasmid

#215887

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-99a-I-type

Plasmid

#215888

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Publication

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)

Expression

Mammalian

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS2

Plasmid

#184885

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Publication

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS3

Plasmid

#184886

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Publication

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS1

Plasmid

#184884

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Publication

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ121-Split-TALED

Plasmid

#218171

Purpose

Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein

Depositor

Yiping Qi

Publication

Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

Insert

TALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e

Use

Plastid base editing

Expression

Plant

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 1161 - 1180 of 1379 results

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