We narrowed to 2,218 results for: dcas9

1. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEVA221T7RNAP

   Plasmid

   #167974

   Purpose

   Expresses T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA221AID-T7RNAP

   Plasmid

   #167977

   Purpose

   Expresses fusion protein AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   AID-T7RNAP
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-EF1alpha-scFv-VP64_Blast

   Plasmid

   #192655

   Purpose

   3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Contains D10A and H840A mutations

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLH-sgRNA1-PP7-boxB

   Plasmid

   #75393

   Purpose

   sgRNA1-PP7-boxB

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7-boxB
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEVA221TrxA-AID-T7RNAP

   Plasmid

   #167976

   Purpose

   Expresses fusion protein TrxA-AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TrxA-AID-T7RNAP
   

   Use

   Tags

   T7RNAP and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGH394

   Plasmid

   #230851

   Purpose

   Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Insert

   rAPOBEC1-dCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCB-CRISPRi

   Plasmid

   #221136

   Purpose

   Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   cbu1169 promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR-U6-gRNA-TRE3G-Nluc

    Plasmid

    #192659

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR-U6-gRNA-miniCMV-Nluc

    Plasmid

    #192657

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Nluc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, miniCMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BB1_23_MS2bind_VP64_SV40NLS

    Plasmid

    #161479

    Purpose

    BB1 encoding a MS2 binding VP64 fusion protein with an additional SV40 NLS

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Insert

    MS2 binding protein + VP64 activation domain
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMM587

    Plasmid

    #133593

    Purpose

    Part Plasmid for dCas9, Part 3b(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM586

    Plasmid

    #133592

    Purpose

    Part Plasmid for dCas9, Part 3(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM729

    Plasmid

    #133598

    Purpose

    Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    Mxi1-dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMM767

    Plasmid

    #133604

    Purpose

    Part Plasmid for dCas9 NLS, Part 3(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM585

    Plasmid

    #133591

    Purpose

    Part Plasmid for dCas9, Part 3a(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM768

    Plasmid

    #133605

    Purpose

    Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM769

    Plasmid

    #133606

    Purpose

    Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

    Depositor

    Megan McClean

    Article

    Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

    Insert

    dCas9 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid

    #85756

    Purpose

    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Inserts

    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    

    Use

    CRISPR

    Tags

    Csy4, Csy4 recognition sequence, FokI fusion via …

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CMV and U6

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge