167,605 results

1. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

   Plasmid

   #168305

   Purpose

   mediated knock-in of sgRNA precursor

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   SaKKH-EGFP-U6-sgRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1alpha, CBh

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. cacna2d1 HA pCAGGS

   Plasmid

   #206107

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV/B-actin

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RabV CVS-N2c(deltaG)-mCherry-P2A-Cre

   Plasmid

   #73472

   Purpose

   Expresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   mCherry-P2A-Cre
   

   Use

   Neurotropic virus

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiT3.1-N_RBBP7:1-425

   Plasmid

   #211113

   Purpose

   Mammalian expression of full-length human RBBP7. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RBBP7:M1-S425 (RBBP7 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-NES-jRGECO1a

   Plasmid

   #61563

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRGECO1a
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS316-4M5.3

   Plasmid

   #45831

   Depositor

   Dane Wittrup

   Insert

   4M5.3
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK033 p5E desma MCS

   Plasmid

   #195948

   Purpose

   Gateway p5E vector

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   desmin a regulatory elements (desma Zebrafish)
   

   Use

   Other

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 puro shSLC2A1-1

   Plasmid

   #193702

   Purpose

   Constitutive lentiviral expression of SLC2A1 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   SLC2A1 (SLC2A1 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR P2R-P3-mKate2

   Plasmid

   #48345

   Purpose

   Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   mKate2
   

   Use

   Gateway entry vector

   Mutation

   Contains a stop codon at the 3' end of the c…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS II KS+ loxP-GFP_ZEO_loxP

    Plasmid

    #128064

    Purpose

    has loxp-GFP-Zeo-Loxp cassette

    Depositor

    Oskar Laur

    Insert

    loxp-GFP-Zeo-Loxp
    

    Use

    Cre/Lox

    Mutation

    no

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-S5E2-C1V1-eYFP (AAV1)

    Viral Prep

    #135633-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-S5E2-C1V1-eYFP (#135633). In addition to the viral particles, you will also receive purified pAAV-S5E2-C1V1-eYFP plasmid DNA. Expression of red-shifted opsin C1V1 fused to EYFP under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Tags

    EYFP

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pDONR P2R-P3-EGFP

    Plasmid

    #48349

    Purpose

    Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Mutation

    Aminoacid 40 is valine in our sequence, while it …

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GGE0029

    Plasmid

    #120730

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDESTmycQKI

    Plasmid

    #19870

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    quaking homolog, KH domain RNA binding (QKI Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

17. CVS-N2c-nl.EGFP-jRGECO1a

    Plasmid

    #172386

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + jRGECO1a
    

    Use

    Neurotropic virus

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ-mgU2-47

    Plasmid

    #127585

    Purpose

    Expresses mgU2-47 scaRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    mgU2-47 scaRNA
    

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. tdTomato-Lysosomes-20

    Plasmid

    #58109

    Purpose

    Localization: Lysosome Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAGM37443

    Plasmid

    #153218

    Purpose

    Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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