169,450 results
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Plasmid#49521PurposeNourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-T38S38-750-luxAB-DAS+4
Plasmid#49518PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertT38S8-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialPromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBOB-VE-cadherin-tdTomato
Plasmid#118733PurposeTo express VE-cadherin tagged with tdTomato on C-terminusDepositorInsertCadherin 5 (CD144) (CDH5 Human)
UseLentiviralTagstdTomatoMutationI503T (Common SNP-see comments)PromoterCMVAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-HF1-plus
Plasmid#126768PurposeExpression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1DepositorInsertSpCas9-HF1-plus
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A; Q695A; Q926A; amino acids 1005-1013 replac…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti EF1a GFP-mCherry-mPlin1
Plasmid#189005PurposeFluorescent reportor for lipophagyDepositorInsertGFP, mChery, Plin1 (Plin1 Mouse)
UseLentiviralAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG-HIV-2LTR
Plasmid#104590Purposestandard for qPCRDepositorInsert2LTR circle junction of HIV-1
UseCloning vectorAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)
Viral Prep#105448-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-29xGGGGCC-12xMS2
Plasmid#99149PurposeEncodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.DepositorInsert29xGGGGCC 12xMS2 hairpins
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRSin_hTCRαγεζ
Plasmid#187351PurposeLentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)DepositorInsertGPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)
UseLentiviralPromoterSFFVAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry
Plasmid#35504PurposeAAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationC128S and D156APromoterEf1aAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Perturb-seq GBC library
Pooled Library#85968PurposeBarcode library for cloning gRNA sublibraries for single cell CRISPR analysis.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-ER
Plasmid#164166PurposeExpresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmCherry2
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mouse b8 full length
Plasmid#217817PurposeFull length mouse integrin b8 expressionDepositorAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-AAVS1-5-1kbdonor
Plasmid#234735PurposePseCAST crRNA targeting AAVS1-5, with 1 kb donor transposonDepositorInsertAAVS1-5 crRNA
ExpressionMammalianAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tet3G blasticidin
Plasmid#128061PurposeLentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
TagsymYPETExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only