We narrowed to 352 results for: tbp
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Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
Tags6His-TEVExpressionBacterialPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q
Plasmid#132545PurposeExpresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM2CCtoSS
Plasmid#89154Purposerecombinant expression of PTBP1-RRM2 C250S,C251S in e.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
TagsIntein and chitin binding domainExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
P6598 MSCV-IP N-HAonly TBPL1
Plasmid#34896DepositorAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTBPL1.1.0-gDNA
Plasmid#112412PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TBPL1DepositorAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBPL1-donor
Plasmid#112316PurposeCRISPR donor plasmid to tag human transcription factor TBPL1 with GFPDepositorInsertTBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
UseCRISPRMutationrs35776966, hg38:6:133987646:GTGTAT>-Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
Tags6His-TEVExpressionBacterialPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE90 pCS2P+ XCtBP
Plasmid#16704DepositorInsertXCtBP (ctbp2 Frog)
UseXenopus expressionAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVLINK-EF1alpha-HA-5'-SETBP1
Plasmid#244266Purpose5' AAVLINK plasmid for SETBP1 expressionDepositorAvailable sinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-tktBp-GFPuv
Plasmid#139081PurposeLow phosphate inducible promoter tktBp for GFP expressionDepositorInserttktBp-GFPuv
ExpressionBacterialAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
Tags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
Tags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
Tags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
Tags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q
Plasmid#132545PurposeExpresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-tktBp-GFPuv
Plasmid#139081PurposeLow phosphate inducible promoter tktBp for GFP expressionDepositorInserttktBp-GFPuv
ExpressionBacterialAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only