We narrowed to 4,739 results for: Drosophila

1. pCFD3-NS

   Plasmid

   #149545

   Purpose

   Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACUH

   Plasmid

   #58374

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han, Yuh-Nung Jan

   Article

   Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUA

   Plasmid

   #58372

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han, Yuh-Nung Jan

   Article

   Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-GMR-eya(shRNA)

   Plasmid

   #157991

   Purpose

   pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

   Depositor

   Greg Beitel

   Article

   Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD5-NS

   Plasmid

   #149546

   Purpose

   Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHACK(Gal4)-DONR(T2A-LexAGAD)

   Plasmid

   #194769

   Purpose

   To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

   Depositor

   Chun Han

   Article

   Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

   Insert

   T2A-LexAGAD
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD3-frame_selector_0

   Plasmid

   #127553

   Purpose

   Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sgRNA frame 0
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. UAS-myr::4xSNAPf

   Plasmid

   #87637

   Purpose

   For creating drosophila transgenics expressing UAS-myr4x::SNAPf

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   myr::4xSNAPf
   

   Expression

   Insect

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD3-frame_selector_1

    Plasmid

    #127554

    Purpose

    Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DmTmem63

    Plasmid

    #136598

    Purpose

    Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    DmTMEM63
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHACK(Gal4)-DONR(T2A-Flp1)

    Plasmid

    #194770

    Purpose

    To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-Flp1
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT57

    Plasmid

    #204303

    Purpose

    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUAST-NachBac-EGFP

    Plasmid

    #40282

    Depositor

    Benjamin White

    Article

    Luan et al J Neurosci. 2006 Jan 11;26(2):573-84.

    Insert

    NaChBac-EGFP
    

    Use

    Drosophila

    Tags

    EGFP

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

15. UAS->Bxb1.STOP>myr::4xSNAPf

    Plasmid

    #87639

    Purpose

    For creating drosophila transgenics expressing UAS->Bxb1.STOP>myr::4xSNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Expression

    Insect

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. UAS-Halo7::CAAX

    Plasmid

    #87645

    Purpose

    For creating drosophila transgenics expressing UAS-Halo7::CAAX

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Halo7::CAAX
    

    Expression

    Insect

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. K560GFP_17NL

    Plasmid

    #129767

    Purpose

    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    DAL insert before A345

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS-7xHalo7::CAAX

    Plasmid

    #87647

    Purpose

    For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    7xHalo7::CAAX
    

    Expression

    Insect

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJFRC7-UAS-NPRR_dTK

    Plasmid

    #133244

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    drosophila tachykinin-fused codon-optimized GCaMP6s
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Rab10

    Plasmid

    #31737

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab10 (Rab10 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only