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Showing: 1 - 20 of 77 results
  1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR... ortholog in the Drosophila genome? Continue reading and I’ll show you how Drosophila can be used to push...favorite gene (YFG) in Drosophila (and even if there’s not!) the wealth of Drosophila genetic tools available..., and overexpress YFG in a Drosophila cell line. Knock downs in Drosophila cell lines are extremely effective...your research in new and exciting directions. Drosophila are very easy to manipulate genetically and have... second post will detail a major tool used by Drosophila geneticists (the Gal4/UAS system), and the third... how you can make your own mutant flies. Find Drosophila Resources at Addgene The birds and the bees ...
  2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...earlier, even genes not found in Drosophila can be studied. Using a Drosophila cell line, I’ve studied a transcription...described the fundamentals of how to work with Drosophila as an experimental model organism. I then described...your flies will be ready for experimentation. Drosophila are amenable to many different kinds of experimental...can be employed to look at oxygen consumption. Drosophila are also used in cancer research (3). The sky..., which flies do not have!) (4). The power of Drosophila as a model organism is in its amenability to ...with flies, you can utilize the resources of the Drosophila community to easily test your hypotheses. Many...had nearly a decade of experience working with Drosophila as an experimental system. Jon enjoys studying...
  3. Cre-ating New Methods for Site-specific Recombination in Drosophila

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    ...this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila, you may be...phenotype. This problem is especially stark in Drosophila, where expression of Cre from the standard UAS...looking to use site-specific recombination in Drosophila, read on to learn about new recombinases suitable...sought to make complex genome modifications in Drosophila using multiple recombinases. To make multiple...Saccharomyces cerevisiae) had been used successfully in Drosophila, so Rubin sought to test the suitability of four...recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for specific recombination patterns...further enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases...
  4. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression of ...be overexpressed or knocked down. To do this, Drosophila geneticists use the Gal4/UAS system. This incredibly...activation system co-opted from yeast (1). It is a Drosophila geneticist’s main workhorse to turn genes on ...had nearly a decade of experience working with Drosophila as an experimental system. Jon enjoys studying...Roman, R. L. Davis, Gene expression systems in Drosophila: A synthesis of time and space, Trends Genet.... : A Versatile Toolkit for Gene Expression in Drosophila, 3, 1–10 (2015). PubMed PMID: 21356876. 5. J....tools for gene expression and clonal analysis in Drosophila, 9, 47–55 (2012). PubMed PMID: 22205518. PubMed...
  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...Wiechaus first identified the hedgehog gene in Drosophila. They observed that flies with non-functional...denticles (bristles) outline the segments of the Drosophila embryo. In flies with non-functional hedgehog...blood vessels). Spaghetti squash (Sqh) is the Drosophila Regulatory Light Chain (RMLC) of nonmuscle myosin... Monster. cookie monster (comr) is actually a Drosophila meiotic arrest gene that is necessary for spermatogenesis...cell fate of the dorsal mesoderm in developing Drosophila embryos (Azplazu and Frasch, 1993).  Tinman, ...determine cell fates in the dorsal mesoderm of Drosophila." Genes & Development 7.7b (1993): 1325-1340....specification of the heart and visceral muscles in Drosophila." Development118.3 (1993): 719-729. PubMed PMID...
  6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    .... So far, tools to overcome this challenge in Drosophila require heat-shocking the system or using chemical...techniques to reliably label any post-mitotic cells in Drosophila. SPARC was developed by Thomas Clandinin’s lab... incorporates several common genetic tools in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor ...labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., ...binding site and effector in the construct. In Drosophila, the SPARC systems can be used both for visualization...tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https://doi.org/10.1038...
  7. Plasmids 101: Repressible Promoters

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    ...as repressible binary systems commonly used in Drosophila. How are repressible promoters regulated? Like...increase QUAS driven expression by ~3,300-fold in Drosophila cells. Co-expression of repressor QS along with... reversible by the addition of quinic acid to Drosophila cells. Work by Wei et al. from Kang Shen’s lab...Refinement of Tools for Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID...mosaic with dual binary transcription systems in Drosophila.” Nat Neurosci 9(5) (2006): 703-9. PubMed PMID...tools for gene expression and clonal analysis in Drosophila.” Nat Methods 9(1) (2011): 47-55. PubMed PMID...the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources...
  8. Five Popular Model Organisms

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    ... The fruit fly (Drosophila melanogaster) Another popular model organism is Drosophila melanogaster, or... Hunt Morgan, who is known as the “father” of Drosophila research. Morgan discovered that genes were found...Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology & medicine." Materials... Charles D. Nichols. "Human disease models in Drosophila melanogaster and the role of the fly in therapeutic...Refinement of tools for targeted gene expression in Drosophila." Genetics 186.2 (2010): 735-755. PubMed PMID... on the Addgene blog Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read...
  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ...study model organisms such as Drosophila and zebrafish. Brainbow in Drosophila has aided in the mapping of...tracing in other systems, such as zebrafish and Drosophila. Why Brainbow? Brainbow highlights the trend ...Clevers H. Cell. 2010 Oct 1;143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling... cell labeling for neural circuit analysis in Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre...
  10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...Labeling Drosophila neurons with dBrainbow and Flybow Based on the Brainbow 1.0 system, Drosophila Brainbow...Brainbow (dBrainbow; Hampel et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that ...Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster and is under the control of Flp ... cell labeling for neural circuit analysis in Drosophila melanogaster." Nature methods 8.3 (2011): 260...
  11. Six Spooky Science Stories and Halloween at Addgene

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    ... a set of cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these... that mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology...
  12. CRISPR 101: Multiplex Expression of gRNAs

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    ...other model systems, including E. coli, plants, Drosophila, and zebrafish!   Why use multiplexed gRNAs?...restriction enzyme BsaI. If you’re working in Drosophila, a two-gRNA expressing plasmid is available from...supplied on a separate plasmid. Multiplexing in Drosophila Bullock Lab Multiplex Plasmid: Two gRNAs can ...or SLIC cloning. gRNAs are expressed from two Drosophila U6 promoters. Cas9 must be supplied on a separate...efficient germline and somatic genome engineering in Drosophila.” Proceedings of the National Academy of Sciences...
  13. Seeing Red: Simple GFP Photoconversion

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    ...simple technique has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use...rapidly and efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible...
  14. Plasmids 101: The Promoter Region – Let's Go!

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    ... from Drosophila Actin 5c gene Constitutive  Commonly used in expression systems for Drosophila. Polyhedrin...transactivator is used. UAS General expression mRNA Drosophila promoter conaining Gal4 binding sites Specific...
  15. Sensing Neuronal Dopamine

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    ...sensors in experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse brain slices and freely...Demonstrate DA detection in a single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening...
  16. Fluorescent Tagging of Endogenous Genes with SapTrap

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    .... They can also be introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR...Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016...
Showing: 1 - 20 of 77 results