167,924 results

1. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

   Plasmid

   #231721

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   Flag-GST-3C

   Expression

   Insect

   Mutation

   RPB1:1586-1971

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO EYFP (AAV8)

   Viral Prep

   #55641-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

3. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   mouse oxytocin

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

   Plasmid

   #225754

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AMPK(T>A)-SPARK

   Plasmid

   #226256

   Purpose

   phospho-null mutant of AMPK-SPARK

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   AMPK(T>A)-SPARK
   

   Expression

   Mammalian

   Mutation

   substituted Threonine 20 with Alanine

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-TFRC

    Plasmid

    #172420

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEZT-BM

    Plasmid

    #74099

    Purpose

    Multi-purpose rapid titer bacmam expression vector

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Bacmam, baculovirus

    Tags

    EGFP following p10 promoter

    Expression

    Mammalian

    Promoter

    CMV, p10

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_mCherry_P2A_Hygro

    Plasmid

    #135003

    Purpose

    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALDH18A1

    Plasmid

    #155402

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV5)

    Viral Prep

    #125712-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Tags

    mScarlet

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

16. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (AAV5)

    Viral Prep

    #209655-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (#209655). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of the high affinity ATP biosensor iATPSnFR2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Promoter

    CAG

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.22-VEGF-HRE::dLUC

    Plasmid

    #128096

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Promoter

    inserted VEGF hypoxia response elements (HRE) (x5)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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