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pAAV-flex-taCasp3-TEVp
(Plasmid #45580)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45580 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5347
  • Total vector size (bp) 7225
  • Vector type
    AAV ; Adeno Associated Viral Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    For liquid cultures grow in LB:2XYT (1:1) overnight at 30 C.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Caspase 3
  • Alt name
    Casp3
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    847
  • Mutation
    Linker replaced with a TEV protease cleavage site
  • Entrez Gene
    CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
  • Promoter EF1a

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer AAA GCA GCG TAT CCA C
  • 3′ sequencing primer AAT CAT GTC CCT GCC GTC GAT C
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Tobacco Etch Virus protease
  • Alt name
    TEV protease
  • Insert Size (bp)
    705
  • Promoter EF1a

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer TTC TCA AGC CTC AGA CAG TGG
  • 3′ sequencing primer AGA GGG GAT CGT TGT AGA AGT C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The taCasp3-T2A-TEVp transgene was generated by overlapping PCR of plasmids harboring taCasp3 and TEVp (Gray et al., 2010; Cell 142, 637-646 PMID: 20723762). This transgene was inserted in reverse orientation into the plasmid pAAV-EF1a-DIO-hChR2(H134R)-EYFP-WPRE-pA such that it replaced hChR2(H134R)-EYFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-flex-taCasp3-TEVp was a gift from Nirao Shah & Jim Wells (Addgene plasmid # 45580 ; http://n2t.net/addgene:45580 ; RRID:Addgene_45580)
  • For your References section:

    Sexually dimorphic neurons in the ventromedial hypothalamus govern mating in both sexes and aggression in males. Yang CF, Chiang MC, Gray DC, Prabhakaran M, Alvarado M, Juntti SA, Unger EK, Wells JA, Shah NM. Cell. 2013 May 9;153(4):896-909. doi: 10.1016/j.cell.2013.04.017. 10.1016/j.cell.2013.04.017 PubMed 23663785