Purpose(Empty Backbone) pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11619||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8500
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Promoter mouse U6
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer mU6-F (5'-ATATCCCTTGGAGAAAAGCCTT-3') (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the mouse U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP.
Please see author's map for more detailed information.
Note: A single base pair deletion at position 11 of the mouse U6 promoter in this plasmid does not impair the efficacy of this reagent.
There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLB was a gift from Stephan Kissler (Addgene plasmid # 11619)
For your References section:In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S, Stern P, Takahashi K, Hunter K, Peterson LB, Wicker LS. Nat Genet. 2006 Apr . 38(4):479-83. 10.1038/ng1766 PubMed 16550170