165,693 results

1. pCasSA

   Plasmid

   #98211

   Purpose

   CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

   Depositor

   Quanjiang Ji

   Article

   Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)

   Viral Prep

   #105558-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   CaMKII

   Tags

   None

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Syn61Δ3(ev5)

   Bacterial Strain

   #174514

   Purpose

   Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Bacterial Resistance

   Streptomycin

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pET-pA-Hia5

   Plasmid

   #174372

   Purpose

   SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein A - hia5
   

   Use

   Tags

   6xHis and Protein A

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+HyPer7-NES

   Plasmid

   #136467

   Purpose

   Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Nuclear export signal from the HIV Rev protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW mCherry-GFP-LC3

   Plasmid

   #110060

   Purpose

   To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

   Depositor

   Anne Brunet

   Article

   Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

   Insert

   mCherry-GFP-LC3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available Since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    7mer insertion in the AAV2 cap

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-pAG-Hia5

    Plasmid

    #174373

    Purpose

    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein AG - hia5
    

    Use

    Tags

    6xHis and Protein A/G

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GFAP-hM4D(Gi)-mCherry (AAV5)

    Viral Prep

    #50479-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-GFAP-hM4D(Gi)-mCherry (#50479). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM4D(Gi)-mCherry plasmid DNA. GFAP-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    GFAP

    Tags

    mCherry

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pDisplay-BirA-ER

    Plasmid

    #20856

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2008 May . 5(5):397-9.

    Insert

    BirA-ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal ER targeting sequence (KDEL)

    Promoter

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest-527

    Plasmid

    #11518

    Purpose

    Gateway destination vector for bacterial expression; His tag

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E_he1a:mCherry

    Plasmid

    #113881

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-mt-mKeima

    Plasmid

    #131626

    Purpose

    lentiviral expression of mitochondrial mKeima for generation of stable cell lines

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-Nesprin3-C-18

    Plasmid

    #54203

    Purpose

    Localization: Nuclear Membrane , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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