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182,051 results

Showing: 1481 - 1500 of 182051 results

  3. dxCas(3.7)-VPR

    Plasmid
    #108383
    Purpose
    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dxCas(3.7)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108383-plasmid-map-sequence-id-207172

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  4. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid
    #84561
    Purpose
    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
    Depositor
    Robert Singer
    Publication
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    Use
    Lentiviral
    Tags
    Flag, Suntag, oxBFP, AID (auxin-inducible degron)
    Promoter
    UbC
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84561-plasmid-map-sequence-id-331246

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  5. mEmerald-N1

    Plasmid
    #53976
    Purpose
    Localization: N1 Cloning Vector, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEmerald
    Expression
    Mammalian
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    53976-plasmid-map-sequence-id-212898

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  6. pEGFP GR

    Plasmid
    #47504
    Purpose
    Expresses glucocorticoid receptor in mammalian cells
    Depositor
    Alice Wong
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Glucocorticoid receptor (NR3C1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47504-plasmid-map-sequence-id-223484

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  7. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid
    #20942
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Insert
    channelrhodopsin-2
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20942-plasmid-map-sequence-id-476966

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  13. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid
    #141385
    Purpose
    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-E (E SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141385-plasmid-map-sequence-id-288068

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  14. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV1)

    Viral prep
    #26969-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  15. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV5)

    Viral prep
    #100837-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60224-plasmid-map-sequence-id-418949

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  19. CMV-B-GECO1

    Plasmid
    #32448
    Purpose
    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Publication
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-GECO1.0
    Expression
    Mammalian
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    CMV
    Available since
    Sept. 30, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32448-plasmid-map-sequence-id-122739

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Showing: 1481 - 1500 of 182051 results