167,945 results

1. V245 pCEP-4HA B56alpha

   Plasmid

   #14532

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
   

   Tags

   4 HA

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn1-tTA

   Plasmid

   #104109

   Purpose

   AAV-mediated expression of tTA under the Syn1 promoter.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOBI-hPLD3-flag

   Plasmid

   #222640

   Purpose

   lentiviral vector for full length human PLD3 expression (with flag tag)

   Depositor

   David Nemazee

   Insert

   PLD3 (PLD3 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL0_9 [pUB110 ORI]

   Plasmid

   #198924

   Purpose

   Level 0 part

   Depositor

   Cultivarium Tools, Charlie Gilbert, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pUB110 ORI
   

   Use

   Synthetic Biology

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-Calr-KDEL-IRES-Puromycin

   Plasmid

   #134664

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   calreticulin (CALR Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 18-413

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEAK10-His-Myc-Kal5

   Plasmid

   #25440

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal5
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. SPARC-bio-His

   Plasmid

   #52078

   Purpose

   Expresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SPARC (SPARC Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFP-HA-DAT

   Plasmid

   #90265

   Purpose

   Expresses RFP-HA-DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Expression

   Mammalian

   Mutation

   inserted HA tag (see YFP-HA-DAT)

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3008

    Plasmid

    #144484

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CDX2 (CDX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTO_CHMP4B_LAP

    Plasmid

    #101850

    Purpose

    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

    Plasmid

    #49771

    Purpose

    Level 1 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    35Sp::hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid

    #128606

    Purpose

    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmNeonGreenHO-G

    Plasmid

    #127912

    Purpose

    a mammalian expression backbone vector for humanized mNeonGreen

    Depositor

    Isei Tanida

    Article

    Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

    Insert

    humanized mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP/golgin-160

    Plasmid

    #180630

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160 (GOLGA3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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