167,785 results

1. lenti-sgRNA blast

   Plasmid

   #104993

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-cPLA2-mEGFP

   Plasmid

   #184718

   Purpose

   Exogenous cPLA2WT

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   cPLA2-mEGFP
   

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-TRX

   Plasmid

   #21615

   Depositor

   John Kyriakis

   Article

   Liu et al Mol Cell Biol. 2000 Mar . 20(6):2198-208

   Insert

   thioredoxin (TXN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221_SLC9A7

   Plasmid

   #132187

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A7 (SLC9A7 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMS158

   Plasmid

   #215679

   Purpose

   Empty repair plasmid for ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2061

   Plasmid

   #141900

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC9A9

   Plasmid

   #132211

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A9 (SLC9A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLenti-PGK-EGFP-ALDH1A3-Neo

   Plasmid

   #189744

   Purpose

   The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKT0178

    Plasmid

    #8744

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG416Gal-GFP

    Plasmid

    #38309

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    EGFP (eGFP )
    

    Expression

    Yeast

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-human-TF-gRNA-puromycin

    Pooled Library

    #162275

    Purpose

    This is a gRNA library targeting on ~1600 human transcription factors for genetic screening.

    Depositor

    Chunliang Li

    Article

    Zhang et al Elife. 2020 Oct 1;9. pii: 57858. doi: 10

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

13. UBC1cer120G

    Plasmid

    #161017

    Purpose

    ubiquitously weakly expressed 120-meric GFP nanocage

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    120meric GFP nanocage
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG416GAL-ccdB-EYFP

    Plasmid

    #14219

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-tetO-Irf4

    Plasmid

    #139804

    Purpose

    doxycycline-inducible expression of mouse Irf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf4 (Irf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAT11

    Plasmid

    #112592

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-smURFP-P2A-GFP

    Plasmid

    #197151

    Purpose

    Expresses the protein of smURFP-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    smURFP-P2A-GFP
    

    Use

    AAV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HSP70-pp7i-mCh-UPG

    Plasmid

    #161011

    Purpose

    C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. iLOV-Farnesyl-5

    Plasmid

    #56516

    Purpose

    Localization: Membrane, Excitation: 450, Emission: 495

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    iLOV

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pAd388-shLuc

    Plasmid

    #83275

    Purpose

    shRNA targeting luciferace

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Promoter

    pUC ori

    Available Since

    Dec. 2, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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