165,637 results

1. pLV-EF1a-mCherry-MinD-IRES-Puro

   Plasmid

   #213112

   Purpose

   Expression of mCherry-MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LRG2.1-BFP

   Plasmid

   #120577

   Purpose

   Lentiviral plasmid to co-express a guide RNA and EBFP.

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. cycLuc_TEVS

   Plasmid

   #119207

   Purpose

   Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with TEVs cleavage site
   

   Use

   Luciferase

   Tags

   6xHis and AU1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJA54 HLA-A*03:01

   Plasmid

   #180451

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*03:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNMT

   Plasmid

   #42578

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   N-myristoyl tranferase (NMT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations T690C and T942C

   Promoter

   T7

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pHdzCas9-KRAB

   Plasmid

   #92341

   Purpose

   Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Type

   Empty backbone

   Use

   CRISPR; Plant expression

   Tags

   KRAB

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-mitfa2.1

   Plasmid

   #81234

   Purpose

   zebrafish mitfa promoter entry vector for multisite Gateway cloning

   Depositor

   James Lister

   Insert

   mitfa promoter (mitfa Zebrafish)
   

   Use

   Multisite gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   mitfa, 2.1kb including 5' UTR

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Vav2

   Plasmid

   #129623

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   Vav2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-FGFR4-V5/HIS

   Plasmid

   #201109

   Purpose

   expression of human FGFR4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-AML1-ETO

    Plasmid

    #12428

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBB-SBP-HA

    Plasmid

    #36100

    Depositor

    Ezra Burstein

    Type

    Empty backbone

    Use

    Tags

    HA and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF2494

    Plasmid

    #142112

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB18 (ZBTB18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-6p-2-EWSR1

    Plasmid

    #46384

    Depositor

    Heinz Gehring

    Article

    Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pLV-EF1a-MinE-EGFP_IRES-Blast

    Plasmid

    #213113

    Purpose

    Expression of MinE-EGFP in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB-mCherry-Giantin

    Plasmid

    #186575

    Purpose

    Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    FRB-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Synthetic, Fission Yeast)
    naa15 (naa15 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1α-DIO-CFL-SN

    Plasmid

    #181740

    Purpose

    AAV vector for expressing CFL-SN

    Depositor

    Yasunori Hayashi

    Article

    Goto et al Science. 2021 Nov 12;374(6569):857-863.

    Insert

    CFL-SN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge