We narrowed to 3,233 results for: multiplex

1. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. P1-EYFP-pA (Construct 5)

   Plasmid

   #55197

   Purpose

   Synthetic promoter responsive to gRNA1

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Ade MLP

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2-ECFP-pA (Construct 6)

   Plasmid

   #55198

   Purpose

   Synthetic promoter responsive to gRNA2

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   mCerulean
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal Ade MLP

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-ExRai-AKAR

   Plasmid

   #118405

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-RAB-ICUE

   Plasmid

   #118480

   Purpose

   ddRFP-based single-color biosensor for monitoring cAMP dynamics.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-ICUE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

   Plasmid

   #210604

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional activator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   hyperdCas12a-miniVPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 8xNFAT-ZsG-mCD8

   Plasmid

   #162747

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-blueAKAR

   Plasmid

   #118470

   Purpose

   cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTA-HUH

    Plasmid

    #221152

    Purpose

    HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Insert

    HisG-URA3-HisG
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLifeAct-miRFP670nano

    Plasmid

    #127434

    Purpose

    F-Actin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    LifeAct-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

    Plasmid

    #59791

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized VP64 dead Cas9 VP64 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2xP53_RE::NLuc

    Plasmid

    #124533

    Purpose

    p53 NLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    P53 NLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMPRAv3:minP-GFP

    Plasmid

    #109036

    Purpose

    MPRA v3 minimal promoter with GFP donor

    Depositor

    Pardis Sabeti, Ryan Tewhey

    Article

    Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

    Insert

    emGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minP

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    VP64 activator fusion; NLS fusion

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TB:7xE-Box:MiniP

    Plasmid

    #133880

    Purpose

    7 copies of the E-box motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies of the E-box motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA5/FRT/TO_TOMM20_HaloTag7

    Plasmid

    #169330

    Purpose

    Expression of TOMM20-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag7 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EKARdual

    Plasmid

    #99870

    Purpose

    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    EKAR
    

    Use

    Tags

    ShadowG and mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AKARdual

    Plasmid

    #99869

    Purpose

    Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    AKAR4
    

    Use

    Tags

    LSSmOrange and mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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