We narrowed to 1,897 results for: sfGFP

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pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221075

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SunTagCACTA1g2-22aa-TET1cd

Plasmid

#106437

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Yeast

Promoter

GPD

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Burden Monitor phi80 version

Plasmid

#66074

Purpose

The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

Plasmid

#48287

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and msfGFP

Expression

Bacterial

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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PCT48

Pooled Library

#170392

Purpose

Barcoded plasmid library

Depositor

Jonathan Weissman

Article

Jones et al Genome Biol. 2020 Apr 14;21(1):92. doi:

Expression

Mammalian

Species

Synthetic

Use

CRISPR and Lentiviral

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only
pUC19_msfGFP-NPM1-repair-template for HCT116

Plasmid

#238245

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1-msfGFP

Plasmid

#174494

Purpose

bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

Sep1 (Sep1 Fly)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:ER-GFP1–10

Plasmid

#125664

Purpose

Binary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Article

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

ER-GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221076

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221077

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221079

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pASPIre4

Plasmid

#196656

Purpose

Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)

Expression

Bacterial

Mutation

SpeI site in Bxb1 CDS

Promoter

rhamnose-inducible promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Strain HK022 JQ929581

Bacterial Strain

#38204

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Bacterial Resistance

Kanamycin

Species

Synthetic

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pKD277

Plasmid

#125098

Purpose

Expression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_2193(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKD287

Plasmid

#125103

Purpose

Expression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4637(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD281

Plasmid

#125100

Purpose

Expression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4428(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKD285

Plasmid

#125102

Purpose

Expression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4623(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKD283.2

Plasmid

#125101

Purpose

Expression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4487(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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