We narrowed to 3,233 results for: multiplex

1. pBlastR-12xLexAOp-mCherry-MiniWhite

   Plasmid

   #165917

   Purpose

   Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable LexAOp Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TDH3 and TEF1

   Available since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCasTns(Vch)-entry(BsaI)

   Plasmid

   #190273

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   no

   Promoter

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAWp30

   Plasmid

   #73857

   Purpose

   pFUGW-EFSp-Cas9-P2A-Zeo

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Insert

   EFSp-Cas9-P2A-Zeo
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)

   Plasmid

   #192122

   Purpose

   Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)

   Depositor

   Wilson Wong

   Article

   Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

   Insert

   pEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-sapphireAKAR

   Plasmid

   #118464

   Purpose

   cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. S9_attL1-EGFP-C-term-attL2

   Plasmid

   #128473

   Purpose

   EGFP entry clone

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   EGFP entry clone
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ141D-gRNA2.1

   Plasmid

   #167161

   Purpose

   Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU3

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKeratin-miRFP670nano

    Plasmid

    #127437

    Purpose

    Keratin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    Keratin-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Keratin V282L - please see depositor comments bel…

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2xp53_RE

    Plasmid

    #118053

    Purpose

    GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

    Plasmid

    #192124

    Purpose

    pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U1

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. STAGR_SAMScaffold_mU6

    Plasmid

    #102845

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163074

    Purpose

    Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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