Skip to main content
Addgene
Showing: 1521 - 1540 of 5038 results

  1. pET3-[Δ1-32]ARL10-His6(CO)

    Plasmid
    #201876
    Purpose
    Used for bacterial expression of human protein ARL10
    Depositor
    Richard Kahn
    Insert
    ARL10 (ARL10 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Deletion of residues 1-32 from N-terminus and cod…
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201876-plasmid-map-sequence-id-403811

    Enlarge

  2. pCCL-c-MNDU3-CD3D-274+5G>A

    Plasmid
    #198023
    Purpose
    LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador
    Depositor
    Donald Kohn
    Insert
    CD3D c.274+5G>A
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Codon optimized (20 bp) on either end of cDNA for…
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198023-plasmid-map-sequence-id-390930

    Enlarge

  3. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

    Enlarge

  4. pHH0103 CEP164 WW domain

    Plasmid
    #104276
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104276-plasmid-map-sequence-id-203766

    Enlarge

  5. pHH0103 IQGAP2 WW domain

    Plasmid
    #104280
    Purpose
    Bacterial expression of WW domain from IQGAP2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP2 WW domain (IQGAP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104280-plasmid-map-sequence-id-203486

    Enlarge

  6. pHH0103 ARHGAP9 WW domain

    Plasmid
    #104286
    Purpose
    Bacterial expression of WW domain from ARHGAP9
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    ARHGAP9 WW domain (ARHGAP9 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104286-plasmid-map-sequence-id-203478

    Enlarge

  7. pHH0103 IQGAP3 WW domain

    Plasmid
    #104289
    Purpose
    Bacterial expression of WW domain from IQGAP3
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    IQGAP3 WW domain (IQGAP3 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104289-plasmid-map-sequence-id-203675

    Enlarge

  8. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104300-plasmid-map-sequence-id-203483

    Enlarge

  9. pHH0103 WWTR1 WW domain

    Plasmid
    #104261
    Purpose
    Bacterial expression of WW domain from WWTR1
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWTR1 WW domain (WWTR1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104261-plasmid-map-sequence-id-203726

    Enlarge

  10. pHH0103 WWOX WW domain #2

    Plasmid
    #104236
    Purpose
    Bacterial expression of WW domain #2 from WWOX
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    WWOX WW domain #2 (WWOX Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104236-plasmid-map-sequence-id-204371

    Enlarge

  11. pJK021

    Plasmid
    #75060
    Purpose
    Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E. coli optimized codons in first 25 residues
    Promoter
    lac
    Available since
    Availability
    Academic Institutions and Nonprofits only
    75060-plasmid-map-sequence-id-142245

    Enlarge

  12. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

    Enlarge

  14. pDIV301

    Plasmid
    #204969
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204969-plasmid-map-sequence-id-454125

    Enlarge

  15. pDIV303

    Plasmid
    #204978
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    amdS
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    two silent mutations (nt177T>C, nt879C>T)
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204978-plasmid-map-sequence-id-415579

    Enlarge

  16. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

    Enlarge

  17. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

    Enlarge

  19. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

    Enlarge

  20. pCMU-4

    Plasmid
    #172353
    Purpose
    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
    Depositor
    Eriko Takano
    Article
    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
    Insert
    D4-TheoR-cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    D4
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172353-plasmid-map-sequence-id-350007

    Enlarge

Showing: 1521 - 1540 of 5038 results