We narrowed to 5,135 results for: codon optimized

1. pSG1039-rA1-SaKKH_P2A_EGFP

   Plasmid

   #239462

   Purpose

   Cytosine base editor with codon-optimized S. aureus KKH Cas9 and rAPOBEC1 deaminase. EGFP can be used as a transfection marker.

   Depositor

   Alexis Komor

   Insert

   S.a. rA1 CBE
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #211343

   Purpose

   Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET3-[Δ1-32]ARL10-His6(CO)

   Plasmid

   #201876

   Purpose

   Used for bacterial expression of human protein ARL10

   Depositor

   Richard Kahn

   Insert

   ARL10 (ARL10 Human)
   

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-32 from N-terminus and cod…

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-c-MNDU3-CD3D-274+5G>A

   Plasmid

   #198023

   Purpose

   LV Expression of CD3D cDNA with mutation at c.274+5G>A used as a disease editing target for CD3d SCID mutation identified in Ecuador

   Depositor

   Donald Kohn

   Insert

   CD3D c.274+5G>A
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized (20 bp) on either end of cDNA for…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCellFree_KA1_SARS-COV-2_ORF7b

   Plasmid

   #169394

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103 CEP164 WW domain

   Plasmid

   #104276

   Purpose

   Bacterial expression of WW domain from CEP164

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   CEP164 WW domain (CEP164 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103 IQGAP2 WW domain

   Plasmid

   #104280

   Purpose

   Bacterial expression of WW domain from IQGAP2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   IQGAP2 WW domain (IQGAP2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103 ARHGAP9 WW domain

   Plasmid

   #104286

   Purpose

   Bacterial expression of WW domain from ARHGAP9

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   ARHGAP9 WW domain (ARHGAP9 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103 IQGAP3 WW domain

   Plasmid

   #104289

   Purpose

   Bacterial expression of WW domain from IQGAP3

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   IQGAP3 WW domain (IQGAP3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 CEP164 WW domain

    Plasmid

    #104300

    Purpose

    Bacterial expression of WW domain from CEP164

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    CEP164 WW domain (CEP164 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103 WWTR1 WW domain

    Plasmid

    #104261

    Purpose

    Bacterial expression of WW domain from WWTR1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWTR1 WW domain (WWTR1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103 WWOX WW domain #2

    Plasmid

    #104236

    Purpose

    Bacterial expression of WW domain #2 from WWOX

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWOX WW domain #2 (WWOX Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK021

    Plasmid

    #75060

    Purpose

    Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Insert

    phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    E. coli optimized codons in first 25 residues

    Promoter

    lac

    Availability

    Academic Institutions and Nonprofits only

    

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14. 141_pT3TS_iCre

    Plasmid

    #171793

    Purpose

    Plasmid for optimised iCre RNA synthesis using T3 promoter.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised Cre for RNA expression
    

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human SLC overexpression library

    Pooled Library

    #213694

    Purpose

    Inducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Akaluc-N1

    Plasmid

    #120371

    Purpose

    Akaluc luciferase mammalian expression in -N1 backbone

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc (human optimized)
    

    Expression

    Mammalian

    Mutation

    Human codon optimized

    Promoter

    CMV

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGDP4 tet(A)

    Plasmid

    #112887

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    tet(A)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence was codon optimized for E. coli K12 usin…

    Promoter

    Plac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIV301

    Plasmid

    #204969

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDIV303

    Plasmid

    #204978

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    amdS
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    two silent mutations (nt177T>C, nt879C>T)

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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