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pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN3_EF1a-tNGFR-2A-Puro

Plasmid

#201113

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 3

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN4_EF1a-tNGFR-2A-Puro

Plasmid

#201114

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 4

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN5_EF1a-tNGFR-2A-Puro

Plasmid

#201115

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 5

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN6_EF1a-tNGFR-2A-Puro

Plasmid

#201116

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 6

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN7_EF1a-tNGFR-2A-Puro

Plasmid

#201117

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 7

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN8_EF1a-tNGFR-2A-Puro

Plasmid

#201118

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 8

Depositor

Pawel Mazur

Publication

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pVH616

Plasmid

#169605

Purpose

Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4 / PPGK

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

Plasmid

#169589

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

Plasmid

#169597

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9 - VP64 fusion

Expression

Mammalian

Promoter

PhCMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1039

Plasmid

#169617

Purpose

Tier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of SEAP and iRFP

Expression

Mammalian

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVH614

Plasmid

#169603

Purpose

Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4 / PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

Plasmid

#169590

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2388-Tier1-PhCMV-p2a_Ypet

Plasmid

#169525

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2389-Tier1-PhCMV-p2a_mRuby

Plasmid

#169526

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1046-Tier1-PhCMV-4xCox8-mRuby2

Plasmid

#169537

Purpose

Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

Plasmid

#169596

Purpose

Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH321-1-Tier1-PhCMV-dCas9-3xNLS

Plasmid

#169595

Purpose

Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Publication

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Expression

Mammalian

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge