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pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pAAV-hSyn-hChR2(H134R)-EYFP

Plasmid

#26973

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

Depositor

Karl Deisseroth

Has service

AAV1, AAV2, AAV5, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

171 Articles

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pAAV-hSyn-hChR2(H134R)-EYFP

Plasmid

#26973

Purpose

AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

Depositor

Karl Deisseroth

Has service

AAV1, AAV2, AAV5, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

171 Articles

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PA-Rac1 (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Publication

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Publication

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pAAV-hSyn-EGFP (AAV8)

Viral prep

#50465-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

Syn

Tags

EGFP

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Publication

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Tags

HA and c-myc

Expression

Yeast

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Publication

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Tags

HA and c-myc

Expression

Yeast

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Publication

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Publication

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pAAV-EF1a-mCherry-IRES-Flpo (AAV Retrograde)

Viral prep

#55634-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. Flpo and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Promoter

EF1a and Ef1a/IRES

Tags

mCherry

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiCRISPRv2

Pooled library

#73632

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles
7TGP

Plasmid

#24305

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-PuroR

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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