179,998 results
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Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-MCS2
Plasmid#46954PurposeCloning plasmid for the generation of Knock-In in human cells using rAAV.DepositorPublicationTypeEmpty backboneUseAAVAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168PurposeCodon optimized Cas9_SV40 NLS with intronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CCSB-Broad Human Kinase ORF Collection
Plasmid kit#1000000014PurposePlasmids for over 500 human kinases and kinase-related proteins in Gateway entry vectors.DepositorApplicationGene Expression and LabelingVector typeMammalian ExpressionAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-mOVA
Plasmid#25099DepositorInsertmembrane bound ovalbumin
ExpressionBacterial and MammalianAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-mSmo
Plasmid#25395DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse Smoothened cDNA (Smo Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROSA26-PA
Plasmid#21271DepositorInsertROSA26 (Gt(ROSA)26Sor Mouse)
UseMouse TargetingAvailable sinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-VHL-pRc/CMV
Plasmid#19999DepositorPublicationInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
TagsHA-tagExpressionMammalianMutationwild typeAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-periHisMBP
Plasmid#11086DepositorTypeEmpty backboneTags6xHis, MBP, and MBP signal peptideExpressionBacterialPromoterTac (lactose/IPTG inducible)Available sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV PHP.eB)
Viral prep#44362-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet_N1
Plasmid#85054PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct
ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
ExpressionBacterialPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CD9-10
Plasmid#55013PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD9 (CD9 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pmAmetrine-DEVD-tdTomato
Plasmid#18879PurposeCaspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
ExpressionMammalianMutationmAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…Available sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M38 TOP-dGFP
Plasmid#17114DepositorInsertTcf/Lef sites (Ctnnb1 )
TagsGFPAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-TLR3-CFP
Plasmid#13641DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertToll-Like Receptor 3 (TLR3 Human)
TagsCFPExpressionMammalianAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-rab7 DN
Plasmid#12660DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAB7 (Homo sapiens) (RAB7A Human)
TagsGFP and HAExpressionMammalianMutationT22NAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by