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pENTR223-EBNALP

Plasmid

#195817

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNALP

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNALP (EBNA-LP Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7a-7

Plasmid

#56443

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab7a (RAB7A Human)

Tags

EGFP

Expression

Mammalian

Mutation

N117S in NM_004637.5

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TFORF0863

Plasmid

#143732

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF2I (GTF2I Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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R777-E352 Hs.TP53-nostop

Plasmid

#70636

Purpose

Gateway ORF clone of human TP53 [NM_000546.5] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

TP53 (TP53 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Nav1.7 Na+ channel scFv [N68/6]

Plasmid

#182094

Purpose

Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRep

Plasmid

#199599

Purpose

Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

Depositor

George Church

Article

Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

Insert

AAV2 rep

Use

AAV

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV-AICD

Plasmid

#69927

Purpose

bacterial expression of MBP-TEV-AICD

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

47 aa long

Promoter

T7

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pAM282

Plasmid

#226121

Purpose

Expression of cdc48

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 (CDC48 Budding Yeast)

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF4

Plasmid

#195811

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF4 (BGLF4 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-AtpU6-26

Plasmid

#160218

Purpose

AtpU6-26 Golden Gate level 0 piece to express gRNAs

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

pAtU6-26 promoter

Use

Golden gate level 0 piece to express grnas

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF5

Plasmid

#198577

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF5 (BALF5 Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_ARP2

Plasmid

#111658

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARP2 (ACTR2 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BRRF2

Plasmid

#195820

Purpose

Gateway Entry Clone of Epstein-Barr virus BRRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRRF2 (BRRF2 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BXLF1

Plasmid

#195825

Purpose

Gateway Entry Clone of Epstein-Barr virus BXLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BXLF1 (BXLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsLSm1_J

Plasmid

#146676

Purpose

Mammalian Expression of HsLSm1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsLSm1 (LSM1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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