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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-TOMM20-N-10

Plasmid

#54282

Purpose

Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pNP3614-MM168 [pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)]

Plasmid

#247266

Purpose

Express ISCro4 recombinase

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)

Expression

Mammalian

Mutation

S30T/P54Q/S243H

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAW62.YY1.FKBP.knock-in.mCherry

Plasmid

#104370

Purpose

For knocking in FKBP F36V P2A mCherry onto YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-mCherry

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpo

Plasmid

#60662

Purpose

Expresses pCAG-Flpo; codon-optimized for mammalian expression

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189925

Purpose

AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Use

AAV

Mutation

SauriCas9 D15A

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Promoter

EF1A

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-tdT (AAV Retrograde)

Viral Prep

#59171-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

Syn

Tags

tdTomato

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only
pB025

Plasmid

#183092

Purpose

Expresses FnCas12a in Bacteroides and used for genome editing

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Insert

FnCas12a, gRNA, Promoter, HAB, TetR,

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P1TDPGH023

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-p65

Plasmid

#111190

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

Plasmid

#216321

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-TGNP-N-10

Plasmid

#54279

Purpose

Localization: Golgi Complex, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TGNP (TGOLN2 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-iLACCO1.2

Plasmid

#218823

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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