168,037 results

1. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Plasmid

   #238237

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS_FtoG
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBItetDMPKSGFP

   Plasmid

   #96903

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Expression

   Mammalian

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK302

   Plasmid

   #87768

   Purpose

   As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Inserts

   PrhaBAD-sfGFP
   rhaS from E. coli with its native RBS from E. coli
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli …

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE8e

   Plasmid

   #138489

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCerulean N1

   Plasmid

   #27795

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   mCerulean N1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-FLAG-hAR

   Plasmid

   #89080

   Purpose

   mammalian expression of FLAG-tagged human androgen receptor

   Depositor

   Elizabeth Wilson

   Article

   Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

   Insert

   hAR (AR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

10. CMV-Tol2 transposase

    Plasmid

    #158774

    Purpose

    Expression of Tol2 transposase

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    Tol2 tranposase
    

    Expression

    Mammalian

    Mutation

    Tol2 codon usage frequency well suitable for muri…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Expression

    Mammalian

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-hOCT3/4

    Plasmid

    #27076

    Purpose

    Non-integrating (episomal) expression of human OCT3/4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-GFP/cre

    Plasmid

    #49054

    Purpose

    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

    Depositor

    Fred Gage

    Article

    Tashiro et al Nat Protoc. 2006;1(6):3049-55.

    Insert

    GFP/Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ABE8.8-m

    Plasmid

    #136294

    Purpose

    expresses ABE8.8-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid

    #140982

    Purpose

    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaQ-RLuc8 (GNAQ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-BE2

    Plasmid

    #73020

    Purpose

    Expresses BE2 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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