Addgene

197,729 results

Showing: 1781 - 1800 of 197729 results

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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra2

Plasmid

#179479

Purpose

Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Expression

Mutation

Promoter

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Expression

Mutation

Promoter

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Farnesyl-5

Plasmid

#55295

Purpose

Localization: Plasma Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Farnesyl (HRAS Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mTagBFP2-Farnesyl-5

Plasmid

#55295

Purpose

Localization: Plasma Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Farnesyl (HRAS Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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CL20_mEGFP_NUP98_KDM5A

Plasmid

#205872

Purpose

Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NUP98_KDM5A (NUP98, KDM5A Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Luc2-Puro)

Plasmid

#80389

Purpose

To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Luc2-F2A-Puromycin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-mCherry

Plasmid

#79422

Purpose

Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK2A1

Plasmid

#23678

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CSNK2A1

Plasmid

#23678

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_hTRIM32

Plasmid

#69541

Purpose

Expresses human TRIM32 fused to c-terminal EGFP

Depositor

Martin Dorf

Article

Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

Insert

tripartite motif containing 32 (TRIM32 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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