161,424 results

1. pZmUBQ:LUC

   Plasmid

   #131860

   Purpose

   Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

   Depositor

   Paul Schulze-Lefert

   Article

   Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

   Insert

   luciferase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ZmUBQ

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-myc-PDGFRbeta

   Plasmid

   #136455

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRB

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRbeta (PDGFRB Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ABE-RA3.0

   Plasmid

   #208301

   Purpose

   Expresses ABE-RA3.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 hygro shRNA E-cadherin

   Plasmid

   #18802

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   E-cadherin shRNA (CDH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Citroff1

   Plasmid

   #134304

   Purpose

   Mammalian expression of inverse-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-RAB35

   Plasmid

   #164630

   Purpose

   Expresses RAB35 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Tags

   cycle 3 GFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   May 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. beta Synuclein-VC

   Plasmid

   #89484

   Purpose

   expresses human beta Synuclein fused to the C-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

   Insert

   SNCB (SNCB Human)
   

   Use

   Tags

   c-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMLS597

   Plasmid

   #188884

   Purpose

   dpy-10 sgRNA expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1-GfaABC1D-lck-jGCaMP8s

   Plasmid

   #176761

   Purpose

   AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Has Service

   AAV1 and AAV9

   Insert

   lck-jGCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-yomKate2-SpHis5

    Plasmid

    #44852

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Rab13

    Plasmid

    #110500

    Purpose

    Fluorescent Rab13

    Depositor

    Bernardo Mainou

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    Rab13 (RAB13 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE Gria1-GFP KI

    Plasmid

    #131489

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-VSVL

    Plasmid

    #64085

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene

    Depositor

    Ian Wickersham

    Insert

    VSV polymerase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP_Rab35 Q67L active

    Plasmid

    #47425

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 Q67L (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Q67L active

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-Luc2-P2A-tdTomato

    Plasmid

    #72486

    Purpose

    Express Luc2 and tdTomato under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1 Lifeact-EGFP

    Plasmid

    #58470

    Purpose

    Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Lifeact
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mouse Chromatin Regulator Library

    Pooled Library

    #200011

    Purpose

    CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

    Depositor

    Bradley Bernstein, Gabriel Griffin, Robert Manguso

    Article

    Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only