|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17343||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)650
Entrez GeneEIF4E (a.k.a. AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
RT-PCR product of mRNA from human A549 cell.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHA-eIF4E was a gift from Dong-Er Zhang (Addgene plasmid # 17343 ; http://n2t.net/addgene:17343 ; RRID:Addgene_17343)
For your References section:ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP. Okumura F, Zou W, Zhang DE. Genes Dev. 2007 Feb 1. 21(3):255-60. 10.1101/gad.1521607 PubMed 17289916