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pcDNA3 HA eIF4GI (1-1599)
(Plasmid #45640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45640 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eIF4GI transcript variant 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4900
  • GenBank ID
    NM_198241
  • Entrez Gene
    EIF4G1 (a.k.a. EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes.

The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA eIF4GI (1-1599) was a gift from Nahum Sonenberg (Addgene plasmid # 45640 ; http://n2t.net/addgene:45640 ; RRID:Addgene_45640)
  • For your References section:

    Requirement of RNA binding of mammalian eukaryotic translation initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E with the mRNA cap. Yanagiya A, Svitkin YV, Shibata S, Mikami S, Imataka H, Sonenberg N. Mol Cell Biol. 2009 Mar;29(6):1661-9. doi: 10.1128/MCB.01187-08. Epub 2008 Dec 29. 10.1128/MCB.01187-08 PubMed 19114555