Purposeratiometric pHluorin2 sequence in a middle entry clone
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73794||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3537
Vector typemiddle entry clone
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameratiometric pHluorin2
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Ratiometric pHluorin2 was originally published in the following paper:
Mahon, M. (2011) pHluorin2: an enhanced, ratiometric, pH-sensitive green florescent protein. Advances in Bioscience and Biotechnology, 2, 132-137. doi: 10.4236/abb.2011.23021.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME pHluorin2 was a gift from David Raible (Addgene plasmid # 73794 ; http://n2t.net/addgene:73794 ; RRID:Addgene_73794)
For your References section:The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity and function. Stawicki TM, Owens KN, Linbo T, Reinhart KE, Rubel EW, Raible DW. Dis Model Mech. 2014 Jul;7(7):847-56. doi: 10.1242/dmm.016576. 10.1242/dmm.016576 PubMed 24973752