167,948 results

1. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

   Plasmid

   #224435

   Purpose

   AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mScarlet-KASH
   U6-gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPR-StAR4GN (Lenti)

   Plasmid

   #222693

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe_Hyg_cOVA_T2A_mStrawberry

   Plasmid

   #161737

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Available Since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Membrane protein gRNA library

   Pooled Library

   #113345

   Purpose

   Contains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAs

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pUCmini-iCAP-PHP.eC

   Plasmid

   #201903

   Purpose

   non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

   Insert

   Synthetic construct isolate AAV-PHP.eC VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CyanoGate Kit

   Plasmid Kit

   #1000000146

   Purpose

   The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

   Depositor

   Alistair McCormick

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

9. spMSP1D1

   Plasmid

   #173482

   Purpose

   Expresses spMSP1D1 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spMSP1D1
   

   Tags

   Histag

   Expression

   Bacterial

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRZero

    Plasmid

    #120275

    Purpose

    Blunt-end cloning vector of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-Flpo (AAV1)

    Viral Prep

    #55637-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    EF1a

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

14. FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries

    Pooled Library

    #1000000228

    Depositor

    Raj Chari

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

    Plasmid

    #236249

    Purpose

    Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV-CuO

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Synthetic, Human)
    

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_nV5-AMOTL1

    Plasmid

    #172998

    Purpose

    constitutive expression of V5-tagged AMOTL1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL1 (AMOTL1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBR1k-GFPuv

    Plasmid

    #106382

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCXLE-hMLN

    Plasmid

    #27079

    Purpose

    Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    C-MYC, LIN28, NANOG (MYC Human)
    

    Expression

    Mammalian

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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