233,958 results

1. Flag-TurboID

   Plasmid

   #124646

   Purpose

   Expresses Flag-TurboID fusion for proximity labeling

   Depositor

   Ken-Ichi Takemaru, FENG-QIAN Li

   Type

   Empty backbone

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP C3-SPG20

   Plasmid

   #218578

   Purpose

   Expresses GFP-tagged human SPG20 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-D1R

   Plasmid

   #89160

   Purpose

   Express vaccinia virus D1R in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D1R
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

   Plasmid

   #196703

   Purpose

   Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 D210N
   

   Expression

   Mammalian

   Mutation

   D210N, catalytically inactive

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-FAST-p10

   Plasmid

   #89152

   Purpose

   Express p10-FAST in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   NBV p10-FAST
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-FAST-p10

    Plasmid

    #89152

    Purpose

    Express p10-FAST in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    NBV p10-FAST
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-pA-Hia5

    Plasmid

    #174372

    Purpose

    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    protein A - hia5
    

    Tags

    6xHis and Protein A

    Expression

    Bacterial

    Available Since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-G-Flamp2-mut

    Plasmid

    #192783

    Purpose

    control probe of G-Flamp2, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2-mut
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV231-xPE

    Plasmid

    #225257

    Purpose

    Mammalian expression of xPE

    Depositor

    Vikash Chauhan, Robert Langer, Phil Sharp

    Article

    Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

    Insert

    xPE
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R221K K848A H982A N1317R

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV Retrograde)

    Viral Prep

    #20297-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Cre-dependent)

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

20. DTA in pCDEST2

    Plasmid

    #223705

    Purpose

    Mammalian expression vector encoding diptheria toxin A

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    Diphtheria toxin A
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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