165,839 results

1. pET15b His6-FKBPF36V

   Plasmid

   #73180

   Purpose

   Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

   Depositor

   Thomas Wandless

   Article

   Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

   Insert

   FKBP(F36V) (FKBP1A Human)
   

   Use

   Tags

   His 6x

   Expression

   Bacterial

   Mutation

   F36V

   Promoter

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

2. ppyCAG_RNaseH1_D210N

   Plasmid

   #111904

   Purpose

   Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N

   Promoter

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV pCAG-FLEX-EGFP-WPRE (AAV1)

   Viral Prep

   #51502-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Promoter

   CAG

   Tags

   EGFP (Cre-dependent)

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pRDA_426

   Plasmid

   #179097

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   ABE8e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG5 Large T

   Plasmid

   #9053

   Depositor

   William Hahn

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Use

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Use

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Promoter

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cdk4-HA

    Plasmid

    #1876

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ChlorON-1

    Plasmid

    #193477

    Purpose

    Turn-on fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

    Insert

    ChlorON-1
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5FRT/Flag-KIAA1267

    Plasmid

    #29758

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    KIAA1267 (KANSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. STK11 gRNA (BRDN0001146194)

    Plasmid

    #75913

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+ Grx1-roCherry

    Plasmid

    #141068

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threon…

    Promoter

    CMV, SP6

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-LMP1-IRES-GFP

    Plasmid

    #220350

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TrkB mEGFP

    Plasmid

    #83952

    Purpose

    FRET donor for dynamically measuring TrkB activity in neurons

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Tropomyosin related kinase B (Ntrk2 Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBbi 41BBL-CD86

    Plasmid

    #183244

    Purpose

    Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    CD86, 41BBL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXspuro-GFP

    Plasmid

    #74203

    Purpose

    Retroviral control vector containing GFP insert

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB533

    Plasmid

    #74872

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcVpx.myc

    Plasmid

    #213388

    Purpose

    Generate lentiviral vector which increases gene delivery into primary human myeloid cells

    Depositor

    Nathaniel Landau

    Article

    Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

    Insert

    SIVmac vpx (vpx Simian Immunodeficiency Virus)
    

    Use

    Lentiviral

    Tags

    myc-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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