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pMA3790
(Plasmid #70110)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70110 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMA2008
  • Backbone manufacturer
    Alexeyev Lab
  • Backbone size w/o insert (bp) 4064
  • Total vector size (bp) 7117
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720
  • Promoter EF1a
  • Tag / Fusion Protein
    • None

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hUNG1 Y147A
  • Alt name
    uracil-N-glycosylase, mitochondrial
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    816
  • Mutation
    Y147A, aa's 1-77 removed
  • Promoter CMV
  • Tags / Fusion Proteins
    • human OTC mitochondrial targeting sequence (N terminal on insert)
    • myc tag (N terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Neo
  • Alt name
    G418 resistance gene, kanamycin resistance gene
  • Insert Size (bp)
    795
  • Promoter SV40 and native

Cloning Information for Gene/Insert 3

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA3790 was a gift from Mikhail Alexeyev (Addgene plasmid # 70110)
  • For your References section:

    Methods for Efficient Elimination of Mitochondrial DNA from Cultured Cells. Spadafora D, Kozhukhar N, Chouljenko VN, Kousoulas KG, Alexeyev MF. PLoS One. 2016 May 2;11(5):e0154684. doi: 10.1371/journal.pone.0154684. eCollection 2016. PONE-D-16-07396 [pii] PubMed 27136098