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pEZT-BM
(Plasmid #74099)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74099 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 74099-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Modified pFastBacDual
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Modifications to backbone
    Polyhedrin promoter removed and replaced with CMV-containing cassette from pVLAD6 vector (Dukkipati et al., Protein Expr Purif., 2008). Multiple cloning site replaced with custom. Synthetic, codon-optimized EGFP inserted after p10 promoter. See Morales-Perez et al., Structure, 2016, for full detail on vector.
  • Vector type
    Mammalian Expression ; Bacmam, Baculovirus
  • Promoter CMV, p10
  • Tag / Fusion Protein
    • EGFP following p10 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Partial sequence from pVLAD6 vector was used in assembling this new expression vector. pVLAD6 was a generous gift from Chris Garcia at Stanford University.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Information for Cloning Grade DNA (Catalog # 74099-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEZT-BM was a gift from Ryan Hibbs (Addgene plasmid # 74099 ; http://n2t.net/addgene:74099 ; RRID:Addgene_74099)
  • For your References section:

    Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins. Morales-Perez CL, Noviello CM, Hibbs RE. Structure. 2016 Mar 24. pii: S0969-2126(16)00082-4. doi: 10.1016/j.str.2016.03.004. 10.1016/j.str.2016.03.004 PubMed 27041595