pEG BacMam
(Plasmid #160451)

• Purpose: (Empty Backbone) vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
• Depositing Lab: Eric Gouaux
• Publication: Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9.

Backbone

• Vector backbone: pFBDM
• Backbone manufacturer: https://www.nature.com/articles/nbt1036
• Modifications to backbone: We chemically synthesized genetic elements derived from the previously described BacMam vector pVLAD (Dukkipati, A., Park, H.H., Waghray, D., Fischer, S. & Garcia, K.C. BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies. Protein Expr. Purif. 62, 160–170 (2008)); a CMV promoter and synthetic intron for efficient RNA splicing and mRNA processing, and a WPRE motif for efficient mRNA processing, stability and export.
• Vector type: Mammalian Expression
• Promoter: CMV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • 1. pEG BacMam.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry