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Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA
Proc Natl Acad Sci U S A. 2013 Aug 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
47867pSimpleII-NLS-NmCas9-HA-NLS(s)This plasmid encode NmCas9 with two NLS and a HA tag
47868pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
47869pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)All-in-one plasmid targeting tdTomato
47870pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.
47871pSTBlue-U6-sgRNA(tdTomato)This plasmid contains expression cassette for sgRNA targeting tdTomato.
47872pSimpleII-NmCas9-FLAGFLAG tagged NmCas9 with no NLS.

Antibodies from Article